Role of retinoids in differentiation and growth of embryonal carcinoma cells.
Role of retinoids in differentiation and growth of embryonal carcinoma cells.
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类维生素A在胚胎癌细胞分化和生长中的作用。
DOI：
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
J. Yuan
中科院分区：
文献类型：
--
作者：
M. Sherman;M. Gubler;U. Barkai;M. Harper;G. Coppola;J. Yuan
为了研究维甲酸如何促进胚胎癌细胞(EC)的分化和抑制其增殖，我们跟踪了它们在细胞内的命运。维甲酸(RA)被许多EC株有效地代谢成更极性的化合物。与RA不同，视黄醇的代谢是缓慢的。我们无法检测到视黄醇转化为视黄酸，这可能表明这两种维甲酸以不同的方式对EC细胞产生影响。添加到培养物中的视黄醇迅速出现在细胞核中；洗涤剂提取后，与细胞核相关的比例最初很低，但随着时间的推移而增加。视黄醇和视黄酸可能通过它们各自的结合蛋白[细胞视黄醇结合蛋白(CRBP)和细胞视黄酸结合蛋白(CRABP)]转运到细胞核：分离的EC核具有与这两种全蛋白而不是其配体的特异的、独立的结合位点。在未经处理的EC细胞的核质中不能检测到CRABP，但在细胞暴露于RA后可以测量到活性。有趣的是，与视黄醇孵育促进CRBP和CRABP进入核质组分。最后，我们证明了短暂的RA暴露会显著降低EC细胞的克隆效率。由于一些细胞即使长期暴露在RA中也不受影响，而它们的后代的生长受到抑制，我们认为EC细胞可能成为RA的表观遗传学难治性细胞。
To study how retinoids promote differentiation and inhibit proliferation of embryonal carcinoma (EC) cells, we have followed their intracellular fate. Retinoic acid (RA) is effectively metabolized to more polar compounds by many EC lines. Unlike RA, retinol is slowly metabolized. Our inability to detect conversion of retinol to RA might indicate that the two retinoids elicit their effects on EC cells in different ways. Retinol added to cultures quickly appears in the nuclear fraction; the proportion associated with nuclei after detergent extraction is initially very low but increases with time. Retinol and RA might be translocated to nuclei by their respective binding proteins [cellular retinol-binding protein (CRBP) and cellular retinoic acid-binding protein (CRABP)]: isolated EC nuclei have specific, independent binding sites for both holoproteins but not their ligands. CRABP cannot be detected in the nucleoplasm of untreated EC cells, but activity is measurable after cells are exposed to RA. Interestingly, incubation with retinol promotes movement of both CRBP and CRABP into the nucleoplasmic fraction. Finally, we have demonstrated that brief exposure to RA dramatically reduces the cloning efficiency of EC cells. Since some cells are unaffected even by lengthy exposures to RA whereas the growth of their progeny is inhibited, we suggest that EC cells can become epigenetically refractory to RA.
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对视黄酸反应改变的 F9 畸胎癌干细胞突变体的选择和表征。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wang,SY;Gudas,LJ
通讯作者：
Gudas,LJ
丁酸盐抑制视黄酸诱导的 F9 畸胎癌干细胞分化。
影响因子：
2.7
作者：
Levine,RA;Campisi,J;Wang,SY;Gudas,LJ
通讯作者：
Gudas,LJ
影响因子：
11.2
作者：
A. Yen;K. Albright
通讯作者：
A. Yen;K. Albright
通过诱导鼠 F9 畸胎癌细胞和人 HL-60 早幼粒细胞白血病细胞的分化来测量一系列新的视黄醛苯甲酸衍生物的结构-活性关系。
影响因子：
11.2
作者：
Strickland,S;Breitman,TR;Frickel,F;Nurrenbach,A;Hadicke,E;Sporn,MB
通讯作者：
Sporn,MB