Independent and coordinate trafficking of single Drosophila germ plasm mRNAs.
Independent and coordinate trafficking of single Drosophila germ plasm mRNAs.
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DOI：
10.1038/ncb3143
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发表时间：
2015-05
影响因子：
21.3
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中科院分区：
文献类型：
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mRNA定位是蛋白质合成空间控制的保守机制，在产生细胞和发育不对称性中起关键作用。虽然不同的转录物可能靶向相同的亚细胞结构域，但它们的定位协调程度尚不清楚。利用定量单分子成像技术，我们分析了果蝇生殖质mRNA颗粒的新生生殖细胞继承的组装。我们发现，生殖细胞注定转录nanos，细胞周期蛋白B，和极性颗粒成分旅行内的卵母细胞的核糖核蛋白颗粒含有单个mRNA分子，但共组装成多拷贝异质颗粒选择性在卵母细胞的后部。组装的化学计量和动力学表明了一个确定的逐步序列。我们的数据表明，这些转录本的共同包装确保了它们有效地分离到生殖细胞。相反，生殖系决定子oskar mRNA的区室化进入不同的颗粒限制了其进入生殖细胞。这种排除是适当的种系发育所必需的。
mRNA localization is a conserved mechanism for spatial control of protein synthesis, with key roles in generating cellular and developmental asymmetry. While different transcripts may be targeted to the same subcellular domain, the extent to which their localization is coordinated is unclear. Using quantitative single molecule imaging, we analyzed the assembly of Drosophila germ plasm mRNA granules inherited by nascent germ cells. We find that the germ cell-destined transcripts nanos, cyclin B, and polar granule component travel within the oocyte as ribonucleoprotein particles containing single mRNA molecules but co-assemble into multi-copy heterogeneous granules selectively at the posterior of the oocyte. The stoichiometry and dynamics of assembly indicate a defined stepwise sequence. Our data suggest that co-packaging of these transcripts ensures their effective segregation to germ cells. In contrast, compartmentalization of the germline determinant oskar mRNA into different granules limits its entry into germ cells. This exclusion is required for proper germline development.