Aspects of cell proliferation in oral epithelial dysplastic lesions
Aspects of cell proliferation in oral epithelial dysplastic lesions
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DOI：
10.1034/j.1600-0714.2000.290201.x
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发表时间：
2000-02-01
影响因子：
3.3
通讯作者：
Felix, DH
中科院分区：
文献类型：
--
作者：
Oliver, RJ;MacDonald, DG;Felix, DH
需要客观的方法来评估口腔上皮癌前病变，并需要可靠的标记物来预测这些病变的恶性变化。使用溴脱氧尿苷 (BrdU) 和 Ki-67 检查舌头和口底 20 个发育不良病变中的细胞增殖，并进行组织学区室分析。每个病例的新鲜活检样本的一半在 BrdU 中孵育 15 分钟，另一半进行常规处理并用于 Ki-67 分析。每个块的切片均用抗 BrdU 和 Ki-67 的抗体进行免疫组织化学染色。根据 Smith & Pindborg 的方法对发育不良进行分级，对使用 Ki-67 评估的 BrdU 标记指数 (LI) 和生长分数 (GF) 进行定量并表示为每毫米基底膜长度 (BL) 和每 100 个总有核细胞 (TNC) 的单位。平均 LI/TNC 为 10.87 (SD 3.65)，平均 LI/BL 为 51.55 (SD 20.75)，平均 GF/TNC 为 26.66 (SD 17.78)，平均 GF/BL 为 157.07 (SD 125.84)。平均上皮厚度为229.09 Cim (SD 104.73)，LI/BL与异型性评分和GF/BL相关，祖细胞区室大小也与异型性评分相关。 BrdU标记指数提供了口腔上皮不典型增生的进一步客观测量，并且祖细胞室很大，这意味着基底细胞增生是不典型增生的重要组成部分。
There is a need for objective methods of assessment of oral epithelial precancerous lesions and reliable markers for the prediction of malignant change in these lesions. Cell proliferation was examined in 20 dysplastic lesions from the tongue and floor of mouth using bromodeoxyuridine (BrdU) and Ki-67, and a histological compartment analysis was performed. Half of a fresh biopsy from each case was incubated in BrdU for 15 min, the other half was routinely processed and used for Ki-67 analysis. Sections from each block were immunohistochemically stained with antibodies against BrdU and Ki-67. Dysplasia was graded according to the method of Smith & Pindborg, The BrdU labelling index (LI) and the growth fraction (GF), assessed by the use of Ki-67, was quantified and expressed as units per millimetre basement membrane length (BL) and per 100 total nucleated cells (TNC). The mean LI/TNC was 10.87 (SD 3.65) and the mean LI/BL was 51.55 (SD 20.75), The mean GF/TNC was 26.66 (SD 17.78) and GF/BL was 157.07 (SD 125.84). The mean epithelial thickness was 229.09 Cim (SD 104.73), The LI/BL correlated with the atypia score and with the GF/BL, The progenitor compartment sizes also correlated with the atypia scores. The BrdU labelling index provides a further objective measurement of oral epithelial dysplasia and the progenitor compartments were large, implying that basal cell hyperplasia is a significant component of the dysplasia.