Reducing the Sampling Rate of Biochemical Measurements Using Fast-Scan Cyclic Voltammetry for In Vivo Applications
Reducing the Sampling Rate of Biochemical Measurements Using Fast-Scan Cyclic Voltammetry for In Vivo Applications
复制标题
DOI：
10.1109/jsen.2014.2321479
复制
发表时间：
2014-09-01
影响因子：
4.3
通讯作者：
McCarty, Gregory S.
中科院分区：
文献类型：
--
作者：
Amos, Alison N.;Roberts, James G.;McCarty, Gregory S.
最近科学技术的进步使无线系统的开发成为可能，这种系统可以在行为正常的动物的功能组织内进行生化测量。然而，数据传输要求和功率限制极大地限制了这些系统的适用性。为了创建可降低传输数据密度和无线系统功耗的协议，本文评估了一种经过验证的活体测量技术--碳纤维微电极的快速扫描循环伏安法(FSCV)的采样率。现有的用于测量大脑中生化信号的FSCV协议是在创建时没有考虑数据密度或功耗的。本文将FSCV检测正常脑组织中神经递质多巴胺的采样频率从10赫兹降低到1赫兹。体外实验表明，1赫兹方案不会对传感器的响应性或选择性产生负面影响。通过直接监测完整脑组织中的多巴胺波动，在体内验证了降低的采样率。与现有协议相比，1赫兹采样率减少了一个数量级的数据量，占空比预计将在无线系统中降低类似的功耗。
Recent advances in science and technology have permitted the development of wireless systems that can make biochemical measurements within functioning tissue in behaving animals. However, data transfer requirements and power limitations have significantly limited the applicability of these systems. In an effort to create protocols that will reduce the density of the data to be transferred and the power consumption of wireless systems, this paper evaluates reducing the sampling rate of a proven in vivo measurement technology, fast-scan cyclic voltammetry (FSCV) at carbon-fiber microelectrodes. Existing FSCV protocols to measure biochemical signaling in the brain were created without consideration for data density or power consumption. In this paper, the sampling rate of the FSCV protocol for detecting the neurotransmitter dopamine in functioning brain tissue was reduced from 10 to 1 Hz. In vitro experiments showed that the 1-Hz protocol did not negatively affect sensor responsivity or selectivity. The reduced sampling rate was verified in vivo by directly monitoring dopamine fluctuations in intact brain tissue. The 1-Hz sampling rate reduces the quantity of data generated by an order of magnitude compared with the existing protocol, and with duty cycling is expected to decrease power consumption by a similar value in wireless systems.