Preferential localization of rat GAPDS on the ribs of fibrous sheath of sperm flagellum and its expression during flagellar formation.
Preferential localization of rat GAPDS on the ribs of fibrous sheath of sperm flagellum and its expression during flagellar formation.
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大鼠GAPD在精子鞭毛纤维鞘肋骨及其在鞭毛形成过程中的表达的优先定位。
DOI：
10.1267/ahc.06020
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发表时间：
2007-02-27
影响因子：
2.4
通讯作者：
Yoshinaga, Kazuya
中科院分区：
文献类型：
--
作者：
Tanii, Ichiro;Yagura, Tetsuya;Inagaki, Naoyuki;Nakayama, Tatsuo;Imaizumi, Kazunori;Yoshinaga, Kazuya
精子鞭毛蛋白的正确组装是精子运动的基础。甘油醛3-磷酸脱氢酶（GAPDS）是精子和精细胞特异性的鞭毛蛋白，是精子鞭毛运动所必需的。为了获得糖酵解酶在鞭毛上的组装信息，我们用单克隆抗体检测了GAPDS在鞭毛上的精确定位和并入鞭毛的阶段。大鼠GAPDS的免疫定位局限于精子鞭毛纤维鞘（FS），且主要分布于肋周而非纵柱。在FS形成的最后一步，在第16步精子的细胞质和鞭毛中首次检测到免疫反应性。结合其他FS蛋白的表达，本研究结果表明了FS组分的顺序组装，提示GAPDS的表达和运输在精子鞭毛形成过程中受到协调调节。
The proper assembly of sperm flagellar proteins is fundamental for sperm motility. The sperm- and spermatid-specific isoform of glyceraldehyde 3-phosphate dehydrogenase, GAPDS, is a flagellar protein indispensable for sperm flagellar movement. To obtain information on the assembly of the glycolytic enzyme into the flagellum, the precise localization of rat GAPDS in the flagellum and the stage of incorporation into the flagellum were examined using a monoclonal antibody. Immunolocalization of rat GAPDS was restricted to the fibrous sheath (FS) in the sperm flagellum, and was predominant in the circumferential ribs rather than the longitudinal columns. Immunoreactivity was first detected in the cytoplasm and flagella of the step-16 spermatids during the final step of FS formation. Together with the expression of other FS proteins, the present results indicate the sequential assembly of FS components, suggesting that the expression and transport of GAPDS is regulated in a coordinated manner during sperm flagellar formation.
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影响因子：
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作者：
Miranda-Vizuete, A;Tsang, K;Oko, R
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