Testes maturation of reared Pacific bluefin tuna Thunnus orientalis at two-plus years old
Testes maturation of reared Pacific bluefin tuna Thunnus orientalis at two-plus years old
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养殖太平洋蓝鳍金枪鱼 Thunnus orientalis 两岁多时睾丸成熟
DOI：
10.1111/j.1444-2906.2007.01438.x
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发表时间：
2007
影响因子：
1.9
通讯作者：
H. Kumai
中科院分区：
文献类型：
--
作者：
Yoshifumi Sawada;M. Seoka;Keitaro Kato;Toshihiro Tamura;Masahiro Nakatani;S. Hayashi;Tokihiko Okada;Kenichi Tose;S. Miyashita;O. Murata;H. Kumai
稳定的繁殖对于向金枪鱼养殖业提供人工孵化的鱼类至关重要。我们观察了太平洋蓝鳍金枪鱼（PBT）东方金枪鱼（Thunnus orientalis）在2岁以上的睾丸成熟。雄鼠睾丸发育成熟的发生率为25.0%，40%的雄鼠睾丸发育中含有精子，而同龄雌鼠的卵母细胞未成熟。1997年8月，这些鱼在0岁以上时被野生捕获，1998年10月和2000年1 - 2月对性腺进行了检查。因此，考虑到野生PBT的产卵季节和捕获时的大小，2000年检查时的年龄估计为2岁7-10个月大。组织学检查显示成熟和发育中的睾丸含有精子、精子细胞、精母细胞和精原细胞。在光镜下观察到所有的精子在海水中都是活动的。根据这项研究和以前的研究结果，在串本，成熟的雄性可能每年至少有5个月是可育的。在年轻的时候在圈养中观察到的睾丸成熟被认为是有希望的，以减少成本的亲鱼维护的少年生产的PBT，特别是如果精子冷冻保存。
Stable reproduction is essential for supplying artificially hatched fish to tuna aquaculture. We observed testes maturation in reared Pacific bluefin tuna (PBT) Thunnus orientalis at 2+ years of age. The incidence of males with mature testes was 25.0%, and 40% of the males had developing testes that contain spermatozoa, while oocytes of the same aged females were not mature. These fish were wild-caught at 0+ years old in August 1997 and the gonads were examined in October 1998 and January–February 2000. Therefore, the age at examination in 2000 was estimated to be 2 years and 7–10 months old considering the spawning season of the wild PBT and the size when captured. Histological examination of thematured and developing testes showed that they contained spermatozoa, spermatids, spermatocytes, and spermatogonia. All the spermatozoa were observed to be motile in sea water under light microscopy. From the results of this and previous studies, matured males are probably fertile for at least 5 months a year in Kushimoto. The testes maturation observed at young age in captivity is considered promising to reduce the cost of brood-stock maintenance for the juvenile production of PBT, especially if the sperm are cryopreserved.
完成太平洋蓝鳍金枪鱼、东方金枪鱼在水产养殖条件下的生命周期
DOI：
--
发表时间：
2005
期刊：
Aquaculture Research 36
影响因子：
--
作者：
M.Inoue;et al.;Yoshitaka Fukada;Ryosuke Watanabe et al.;Sawada Y. et al.
通讯作者：
Sawada Y. et al.