Humidity Control Strategies for Solid-State Fermentation: Capillary Water Supply by Water-Retention Materials and Negative-Pressure Auto-controlled Irrigation
Humidity Control Strategies for Solid-State Fermentation: Capillary Water Supply by Water-Retention Materials and Negative-Pressure Auto-controlled Irrigation
复制标题
固态发酵湿度控制策略：保水材料毛细管供水和负压自动控制灌溉
DOI：
10.3389/fbioe.2019.00263
复制
发表时间：
2019-10-17
影响因子：
5.7
通讯作者：
Gu, Jiayu
中科院分区：
文献类型：
--
作者：
He, Qin;Peng, Huadong;Gu, Jiayu
固态发酵由于其在固体废物处理和发酵工业中的优势而重新受到人们的关注。然而，非均质传热和传质往往是由于缺乏自由水和显着的水分损失，从微生物利用和水分蒸发SSF。基质中水分主要以毛细水的形式存在和流失，因此有必要在毛细水在线监测的基础上探索更有效的方法来解决SSF的失水和补水问题。提出了两种新的毛细管供水策略，建立和评价使用三个选定的参考菌株，包括保水材料和负压自控灌溉（NPACI）。本研究采用保水材料高吸水性聚合物提高酶产率，最大提高幅度为2.47倍。此外，NPACI和0.1%高吸水性聚合物的组合将生产率提高了2.80倍，同时降低了温度、湿度和产品的梯度。此外，一个修改后的液体供应SSF构建通过成功的毛细水控制所提出的湿度控制策略。这种改良的SSF系统可以解决传统SSF培养不均匀的缺点。
Solid-state fermentation (SSF) has regained interest owing to its advantages in solid waste treatment and fermentation industries. However, heterogeneous heat and mass transfer are often caused by the absence of free water and noticeable water loss from microbial utilization and moisture evaporation in SSF. It is necessary to explore more effective ways to solve issues of water loss and water supplement in SSF based on online capillary water monitoring, because capillary water is the dominant form of water that is present and lost in substrate. Two novel capillary-water supply strategies were proposed, established and evaluated using three selected reference strains, including water-retention materials and negative-pressure auto-controlled irrigation (NPACI). This study employed superabsorbent polymer, a kind of water-retention material to enhance enzyme productivity with the most significant increase of 2.47 times. Moreover, the combination of NPACI and 0.1% superabsorbent polymers increased productivity by 2.80-fold, together with lowered gradients of temperature, moisture and products. Furthermore, a modified liquid-supply SSF was constructed through successful capillary water control by proposed humidity control strategies. This modified SSF system could address the shortcomings of inhomogeneous culture of traditional SSF.