An Integrative Investigation ofParabistichella variabilis(Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny
An Integrative Investigation ofParabistichella variabilis(Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny
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多变拟拟菌（原生纲、纤毛纲、少毛纲）的一般形态、超微结构、个体发生和分子系统发育的综合研究
DOI：
10.1111/jeu.12809
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发表时间：
2020
影响因子：
2.2
通讯作者：
Warren Alan
中科院分区：
文献类型：
--
作者：
Dong Jingyi;Chen Xumiao;Liu Yongqiang;Ni Bing;Fan Xinpeng;Li Lifang;Warren Alan
Hypotrichs是一种高度分化和非常多样化的纤毛原生生物。它们的系统学和分类学具有挑战性，需要对其一般形态、超微结构、个体发育和分子生物学进行详细的研究。在这里，一个全面的研究进行了微咸水人口ofParabistichella variabilis使用光学和电子显微镜和系统发育分析的基础上小亚基核糖体DNA序列数据。它的形态，包括纤毛下，表膜，核，颊密封，和extrusomes，记录。目前的研究结果表明，inP. variabilis：（i）皮层颗粒是外泌体，这与其他hypothrichs不同;（ii）颊密封由质膜界定，并包含一个单一的纵向微管层;（iii）可能存在两个收缩空泡，而不是一个;和（iv）咽盘由一个单一的膜界定。第一次描述了个体发育的早期到中期阶段，使这一过程的完整表征成为可能。系统发育分析表明，变异副盾甲藻与短直双端藻、大颗粒尾纤藻和Pellionellum速体藻等不同属的几个种关系密切。然而，需要更多的分类群的超微结构和基因序列数据，以解决系统学的Parabistichella。
Hypotrichs are a highly differentiated and very diverse group of ciliated protists. Their systematics and taxonomy are challenging and call for detailed investigations on their general morphology, ultrastructure, ontogenesis, and molecular phylogeny. Here, a comprehensive study is conducted on a brackish water population ofParabistichella variabilisusing light and electron microscopy and phylogenetic analyses based on small subunit ribosomal DNA sequence data. Its morphology, including the infraciliature, pellicle, nuclei, buccal seal, and extrusomes, is documented. The present findings indicate that inP. variabilis: (i) the cortical granules are extrusomes, which differ from those of other hypotrichs; (ii) the buccal seal is bounded by the plasma membrane and contains a single layer of longitudinal microtubules; (iii) two contractile vacuoles might be present rather than one; and (iv) the pharyngeal disks are bounded by a single membrane. Early‐to‐middle stages of ontogenesis are described for the first time, enabling the complete characterization of this process. Phylogenetic analyses indicate thatParabistichella variabilisis closely related to several species from different genera, such asOrthoamphisiella breviseries,Uroleptoides magnigranulosus, andTachysoma pellionellum. However, ultrastructural and gene sequence data for more taxa are needed in order to resolve the systematics ofParabistichella.