An evolutionarily conserved motif is required for Plasmodesmata-located protein 5 to regulate cell-to-cell movement
An evolutionarily conserved motif is required for Plasmodesmata-located protein 5 to regulate cell-to-cell movement
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DOI：
10.1038/s42003-020-1007-0
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发表时间：
2020-06
影响因子：
5.9
通讯作者：
Xu Wang;Gabriel Robles Luna;C. Arighi;Jung-Youn Lee
中科院分区：
文献类型：
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作者：
Xu Wang;Gabriel Robles Luna;C. Arighi;Jung-Youn Lee
许多细胞表面受体和受体样蛋白（RLP）通过跨膜结构域（TMD）进行激活或失活。植物RLP的一个子集独特地定位于被称为胞间连丝的质膜衬里的孔中。这些RLP包括拟南芥胞间连丝定位蛋白（PDLP）5，它以其调节胞间连丝门控和细胞间分子运动的重要功能而闻名。在这项研究中，我们报告说，TMD，虽然不是一个决定性因素的胞间连丝靶向，为PDLP 5功能的重要作用。除了其膜锚定作用外，TMD还介导PDLP 5自身相互作用，并携带PDLP 5调节细胞间运动所必需的进化保守基序。基于计算建模的分析表明，PDLP TMD具有高的二聚化倾向。我们讨论了TMD二聚化的特定模式如何作为PDLP 5和其他PDLP成员调节细胞间运动的共同机制。
Numerous cell surface receptors and receptor-like proteins (RLPs) undergo activation or deactivation via a transmembrane domain (TMD). A subset of plant RLPs distinctively localizes to the plasma membrane-lined pores called plasmodesmata. Those RLPs include theArabidopsis thalianaPlasmodesmata-located protein (PDLP) 5, which is well known for its vital function regulating plasmodesmal gating and molecular movement between cells. In this study, we report that the TMD, although not a determining factor for the plasmodesmal targeting, serves essential roles for the PDLP5 function. In addition to its role for membrane anchoring, the TMD mediates PDLP5 self-interaction and carries an evolutionarily conserved motif that is essential for PDLP5 to regulate cell-to-cell movement. Computational modeling-based analyses suggest that PDLP TMDs have high propensities to dimerize. We discuss how a specific mode(s) of TMD dimerization might serve as a common mechanism for PDLP5 and other PDLP members to regulate cell-to-cell movement.