Transcriptome profiling of postharvest strawberry fruit in response to exogenous auxin and abscisic acid
Transcriptome profiling of postharvest strawberry fruit in response to exogenous auxin and abscisic acid
复制标题
采后草莓果实对外源生长素和脱落酸的转录组分析
DOI：
10.1007/s00425-015-2402-5
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发表时间：
2016-01-01
期刊：
影响因子：
4.3
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
--
作者：
Chen, Jingxin;Mao, Linchun;Luo, Zisheng
生长素和脱落酸通过信号转导通路的串扰调控草莓果实的成熟和衰老，进而调控果实理化性状相关的结构基因。分析了IAA、ABA及其组合对草莓果实生理和转录组学的影响。外源IAA延迟草莓采后成熟，ABA促进采后成熟。然而，IAA和ABA组合处理既没有减缓也没有加速草莓果实的采后成熟。在分子水平上，外源IAA上调了IAA信号相关基因的表达，包括AUX/IAA、ARF、toppless以及编码E3泛素蛋白连接酶和膜联蛋白的基因，下调了果胶解聚、细胞壁降解、蔗糖和花青素生物合成相关基因的表达。相比之下，外源ABA诱导了果实软化相关基因，以及参与信号通路的基因，包括SKP1、HSPs、CK2和SRG1。比较IAA和ABA单独或联合处理对果实转录组的响应，发现IAA和ABA在果实中既有合作作用，也有拮抗作用。然而，在IAA和ABA联合响应的差异表达基因中，有17%是唯一的，而在单独响应IAA或ABA的差异表达基因中没有发现。分析还发现，受体样激酶和泛素连接酶对IAA和ABA都有反应，这似乎在两种激素的信号通路中起着关键作用，因此可能是两种激素的交叉点。
Auxin and abscisic acid regulate strawberry fruit ripening and senescence through cross-talk of their signal transduction pathways that further modulate the structural genes related to physico-chemical properties of fruit.The physiological and transcriptomic changes in harvested strawberry fruits in responses to IAA, ABA and their combination were analyzed. Exogenous IAA delayed the ripening process of strawberries after harvest while ABA promoted the postharvest ripening. However, treatment with a combination of IAA and ABA did not slow down nor accelerate the postharvest ripening in the strawberry fruits. At the molecular level, exogenous IAA up regulated the expressions of genes related to IAA signaling, including AUX/IAA, ARF, TOPLESS and genes encoding E3 ubiquitin protein ligase and annexin, and down regulated genes related to pectin depolymerization, cell wall degradation, sucrose and anthocyanin biosyntheses. In contrast, exogenous ABA induced genes related to fruit softening, and genes involved in signaling pathways including SKP1, HSPs, CK2, and SRG1. Comparison of transcriptomes in responses to individual treatments with IAA or ABA or the combination revealed that there were cooperative and antagonistic actions between IAA and ABA in fruit. However, 17 % of the differentially expressed unigenes in response to the combination of IAA and ABA were unique and were not found in those unigenes responding to either IAA or ABA alone. The analyses also found that receptor-like kinases and ubiquitin ligases responded to both IAA and ABA, which seemed to play a pivotal role in both hormones' signaling pathways and thus might be the cross-talk points of both hormones.