Corneal Inflammation After Miniature Keratoprosthesis Implantation
Corneal Inflammation After Miniature Keratoprosthesis Implantation
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DOI：
10.1167/iovs.14-15884
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发表时间：
2015-01-01
影响因子：
4.4
通讯作者：
Dana, Reza
中科院分区：
文献类型：
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作者：
Crnej, Alja;Omoto, Masahiro;Dana, Reza
目的。比较同种异体穿透性角膜移植(PK)和微型人工角膜(m-KPro)植入后小鼠的角膜炎症反应。方法将BALB/C(同基因)或C57BL/6(异体)角膜作为PK或m-KPro植入的一部分移植到BALB/C宿主床上。在移植后2、4、8周用流式细胞仪检测CD45(+)白细胞、CD4(+)T细胞、CD11b(+)细胞和Gr-1(+)粒细胞/单核细胞的比例来评价角膜炎症反应。结果：同基因(Syn)和异基因(Allo)m-KPro组在各时间点的细胞频率分别高于同基因和异基因PK组。然而，在第4周后，allPK组所有分析的免疫细胞的频率都高于synKPro组。8周时，synKPro组、allPK组和allKPro组的肿瘤坏死因子-α的表达均高于naive组和synPK组。结论：虽然m-KPro装置植入后增强了角膜的炎症反应，但(载体组织的)同种异体反应也是导致角膜炎症的重要因素。这些数据表明，使用同基因或去细胞的角膜组织作为Boston-KPro载体可以减少术后的炎症反应。
PURPOSE. To compare corneal inflammation after syngeneic and allogeneic penetrating keratoplasty (PK) with miniature Keratoprosthesis (m-KPro) implantation in mice.METHODS. BALB/C (syngeneic) or C57BL/6 (allogeneic) corneas were transplanted onto BALB/C host beds as part of PK or m-KPro implantation. Corneal inflammation was assessed by determining the frequencies of CD45(+) leukocytes, CD4(+) T cells, CD11b(+) cells, and Gr-1(+) granulocytes/monocytes by flow cytometry at 2, 4, and 8 weeks post transplantation. In addition, expression levels of the proinflammatory cytokines TNF-alpha and IL-1 beta were analyzed using real-time qPCR at 8 weeks post transplantation.RESULTS. Cell frequencies in the syngeneic (syn) and allogeneic (allo) m-KPro groups were higher compared with the syngeneic and allogeneic PK groups, respectively, at all time points. However, after week 4, frequencies of all analyzed immune cells were higher in the alloPK group as compared with synKPro group. At 8 weeks, the expression of TNF-alpha was higher in synKPro, alloPK, and alloKPro groups compared with the naive and synPK groups. The expression of IL-1 beta was significantly higher in both KPro groups as compared with PK groups.CONCLUSIONS. Although the m-KPro device augments the inflammatory response in the cornea after its implantation, allogenicity (of the carrier tissue) is also a significant contributor to corneal inflammation. These data suggest that using syngeneic or decellularized corneal tissue as a Boston-KPro carrier could reduce the postoperative inflammation response.