Allergy to human seminal fluid:: Cross-reactivity with dog dander
Allergy to human seminal fluid:: Cross-reactivity with dog dander
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DOI：
10.1016/j.jaci.2007.10.008
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发表时间：
2008-01-01
影响因子：
14.2
通讯作者：
Cistero-Bahima, Anna
中科院分区：
文献类型：
--
作者：
Basagana, Maria;Bartolome, Borja;Cistero-Bahima, Anna
背景：人精浆（HSP）过敏并不常见，症状包括外阴阴道瘙痒到危及生命的过敏反应。尽管已报道了几种精浆过敏原，并且其分子质量估计在 12 至 75 kd 之间，但前列腺特异性抗原 (PSA) 最近已被确定为致病过敏原。鉴于许多病例在第一次性交期间或之后出现症状，可能涉及交叉反应现象。 目的：我们试图评估狗上皮蛋白和 HSP 之间是否存在 IgE 交叉反应性，并试图识别所涉及的过敏原。 方法：选择了 41 名狗上皮过敏患者。其中一位在接触丈夫的精浆后出现了过敏反应。进行皮肤点刺试验、血清特异性 IgE 测量、SDS-PAGE 免疫印迹和抑制试验来研究 IgE 结合蛋白的模式以及 HSP 和狗上皮之间的潜在交叉反应性。通过质谱分析来鉴定参与过敏反应的蛋白质。结果：24% 的狗上皮过敏患者血清在 HSP 免疫印迹中识别出 28 kd 的 IgE 结合带。质谱分析将该条带鉴定为 PSA。当这些患者的血清与狗皮屑提取物预孵育时，SDS-PAGE 免疫印迹抑制显示出完全的 IgE 结合抑制。结论：狗皮屑和人 PSA 的蛋白质之间存在 IgE 交叉反应性。
Background: Human seminal plasma (HSP) allergy is uncommon, with symptoms ranging from vulvovaginal pruritus to life-threatening anaphylaxis. Although several seminal plasma allergens have been reported and their molecular masses have been estimated to range between 12 and 75 kd, the prostate-specific antigen (PSA) has recently been identified as a causative allergen. Given that in a large number of cases symptoms appeared during or after the first intercourse, a cross- reactivity phenomenon might be implicated.Objective: We sought to assess the presence of IgE cross-reactivity among proteins from dog epithelium and HSP and to attempt to identify the allergens involved.Methods: Forty-one patients with dog epithelium allergy were selected. One of them experienced anaphylaxis in contact with her husband's seminal plasma. Skin prick tests, serum specific IgE measurements, SDS-PAGE immunoblotting, and inhibition tests were performed to study the pattern of IgE-binding proteins and the potential cross-reactivity between HSP and dog epithelium. Mass spectrometry was carried out to identify the protein involved in allergy reactions.Results: Twenty-four percent of the sera from patients with dog epithelium allergy recognized an IgE-binding band of 28 kd in HSP immunoblotting. Mass spectrometry identified this band as the PSA. SDS-PAGE immunoblotting-inhibition showed a complete IgE-binding inhibition when sera from these patients were preincubated with dog dander extract.Conclusions: IgE cross- reactivity among proteins from dog dander and human PSA is demonstrated.