The overexpression and prognostic role of DCAF13 in hepatocellular carcinoma
The overexpression and prognostic role of DCAF13 in hepatocellular carcinoma
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DCAF13在肝细胞癌中的过度表达及其预后作用
DOI：
10.1177/1010428317705753
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发表时间：
2017-06-20
期刊：
影响因子：
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通讯作者：
He, Xiaodong
中科院分区：
文献类型：
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作者：
Cao, Jianzhong;Hou, Pengjiao;He, Xiaodong
DDB1和CUL4相关因子13(DCAF13)是位于染色体8q22.3上的一个蛋白质编码基因，是多种肿瘤中扩增的热点。据报道，DCAF13在乳腺癌患者中经常被扩增。然而，DCAF13在包括肝细胞癌在内的其他癌症中的基因改变和潜在作用尚未被研究。在这项研究中，我们发现DCAF13在14.7%的病例中被扩增，其在癌症基因组图谱数据集中的肝细胞癌样本中的表达上调(p<；0.001)。DCAF13在40对肝细胞癌和癌旁非肿瘤组织中的表达在信使核糖核酸和蛋白水平均升高(p分别为0.0002和0.0016)。DCAF13表达升高与肿瘤分级呈正相关(p=0.005)，DCAF13表达增高的肝癌患者的生存期明显低于DCAF13表达降低的肝癌患者(中位生存期分别为45.73月和70.53月)。多因素Cox回归分析显示，DCAF13是影响肝细胞癌患者生存的独立预后因素。基因本体论和京都百科全书的基因和基因组分析表明，DCAF13作为一个重要的细胞周期调节因子具有潜在的作用。综上所述，我们的研究结果显示，DCAF13在肝细胞癌中的过度表达与患者的生存率显著相关，并可能参与细胞周期进程的调节。
DDB1 and CUL4 associated factor 13 (DCAF13) is a protein coding gene located on chromosome 8q22.3, which is a hotspot amplified in various cancers. DCAF13 has been reported to be frequently amplified in breast cancer patients. However, the genetic alteration and potential role of DCAF13 in other cancers, including hepatocellular carcinoma, have not been investigated yet. In this study, we found that DCAF13 was amplified in 14.7% of the cases and its expression was upregulated (p < 0.001) in hepatocellular carcinoma samples in The Cancer Genome Atlas dataset. Increased expression of DCAF13 was also noticed in 40 paired hepatocellular carcinoma and adjacent non-tumor tissues both at messenger RNA and protein levels (p = 0.0002 and 0.0016, respectively). A positive relationship was observed between augmented DCAF13 levels and poorer tumor grade (p = 0.005), and we also found that hepatocellular carcinoma patients with increased DCAF13 expression in their tumors had significantly poorer survival compared with those with decreased DCAF13 expression (median survival time: 45.73 and 70.53 months, respectively). Multivariate Cox regression analysis showed that DCAF13 was an independent prognostic predictor of survival in hepatocellular carcinoma patients. Gene ontology and Kyoto Encyclopedia of Genes and genomes analysis indicated the potential role of DCAF13 as a crucial cell cycle regulator. Collectively, our findings revealed that the overexpression of DCAF13 in hepatocellular carcinoma was significantly associated with poor survival and may participate in the regulation of cell cycle progression.