A Novel Surface Antigen of Relapsing Fever Spirochetes Can Discriminate between Relapsing Fever and Lyme Borreliosis
A Novel Surface Antigen of Relapsing Fever Spirochetes Can Discriminate between Relapsing Fever and Lyme Borreliosis
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DOI：
10.1128/cvi.00518-09
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发表时间：
2010-04-01
影响因子：
--
通讯作者：
Schwan, Tom G.
中科院分区：
文献类型：
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作者：
Lopez, Job E.;Schrumpf, Merry E.;Schwan, Tom G.
在先前的赫氏疏螺旋体的免疫蛋白质组分析中，鉴定了结合来自感染回归热螺旋体的小鼠和患者的IgM抗体的候选抗原。一个候选人，被确定为一个假设的蛋白质的分子量为57 kDa，我们已经指定疏螺旋体免疫原性蛋白A（BPA）。进一步研究了该蛋白作为B的潜在诊断抗原。hermsii，因为它不存在于伯氏疏螺旋体基因组中。对39株B的bipA基因进行扩增和测序。hermsii是从北美西部获得的。bipA也表达为重组融合蛋白。感染B的小鼠和患者的血清样本。hermsii或B. burgdorferi的免疫原性，以证实重组蛋白在回归热螺旋体感染的患者中的免疫原性。最后，计算机模拟和实验分析表明，在B中，BipA是表面暴露的脂蛋白。赫姆斯。这些发现提高了诊断回归热螺旋体感染的能力。
In a previous immunoproteome analysis of Borrelia hermsii, candidate antigens that bound IgM antibodies from mice and patients infected with relapsing fever spirochetes were identified. One candidate that was identified is a hypothetical protein with a molecular mass of 57 kDa that we have designated Borrelia immunogenic protein A (BipA). This protein was further investigated as a potential diagnostic antigen for B. hermsii given that it is absent from the Borrelia burgdorferi genome. The bipA locus was amplified and sequenced from 39 isolates of B. hermsii that had been acquired from western North America. bipA was also expressed as a recombinant fusion protein. Serum samples from mice and patients infected with B. hermsii or B. burgdorferi were used to confirm the immunogenicity of the recombinant protein in patients infected with relapsing fever spirochetes. Lastly, in silico and experimental analysis indicated that BipA is a surface-exposed lipoprotein in B. hermsii. These findings enhance the capabilities of diagnosing infection with relapsing fever spirochetes.