Processed xenogenic cartilage as innovative biomatrix for cartilage tissue engineering: effects on chondrocyte differentiation and function
Processed xenogenic cartilage as innovative biomatrix for cartilage tissue engineering: effects on chondrocyte differentiation and function
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DOI：
10.1002/term.1650
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发表时间：
2015-12-01
影响因子：
3.3
通讯作者：
Rotter, Nicole
中科院分区：
文献类型：
--
作者：
Schwarz, Silke;Elsaesser, Alexander F.;Rotter, Nicole
开发用于重建和置换软骨缺损的新生物植入物基质的一个关键点是提供足够的微环境以确保软骨细胞迁移和软骨特异性细胞外基质（ECM）的从头合成。最近开发的脱细胞和灭菌过程保持了天然中隔软骨的三维（3D）胶原结构，同时增加了基质孔隙率，这被认为是软骨组织工程的关键。人原代鼻中隔软骨细胞在单层培养中扩增，并在处理过的猪鼻中隔软骨支架上进行三维培养。在蛋白质和基因表达水平上检查软骨形成生长因子对ECM蛋白新合成的影响。接种实验表明，处理的异种软骨基质提供了优良的环境特性，人鼻中隔软骨细胞的细胞粘附，迁移到基质和软骨特异性ECM蛋白，如II型胶原蛋白和聚集蛋白聚糖的新合成。基质生物力学稳定性表明，在长达42天的3D培养过程中，构建体恢复了完全的稳定性和功能，与II型胶原和GAG的产生成比例。因此，加工异种软骨提供了一个合适的环境，为人类鼻软骨细胞和有前途的潜力，在头颈部的软骨组织工程。版权所有（C）2012约翰威利父子有限公司
One key point in the development of new bioimplant matrices for the reconstruction and replacement of cartilage defects is to provide an adequate microenvironment to ensure chondrocyte migration and de novo synthesis of cartilage-specific extracellular matrix (ECM). A recently developed decellularization and sterilization process maintains the three-dimensional (3D) collagen structure of native septal cartilage while increasing matrix porosity, which is considered to be crucial for cartilage tissue engineering. Human primary nasal septal chondrocytes were amplified in monolayer culture and 3D-cultured on processed porcine nasal septal cartilage scaffolds. The influence of chondrogenic growth factors on neosynthesis of ECM proteins was examined at the protein and gene expression levels. Seeding experiments demonstrated that processed xenogenic cartilage matrices provide excellent environmental properties for human nasal septal chondrocytes with respect to cell adhesion, migration into the matrix and neosynthesis of cartilage-specific ECM proteins, such as collagen type II and aggrecan. Matrix biomechanical stability indicated that the constructs retrieve full stability and function during 3D culture for up to 42 days, proportional to collagen type II and GAG production. Thus, processed xenogenic cartilage offers a suitable environment for human nasal chondrocytes and has promising potential for cartilage tissue engineering in the head and neck region. Copyright (C) 2012 John Wiley & Sons, Ltd.