Label-Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes.
Label-Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes.
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DOI：
10.1002/advs.201600522
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发表时间：
2017-05
期刊：
影响因子：
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通讯作者：
Khademhosseini A
中科院分区：
文献类型：
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作者：
Shin SR;Kilic T;Zhang YS;Avci H;Hu N;Kim D;Branco C;Aleman J;Massa S;Silvestri A;Kang J;Desalvo A;Hussaini MA;Chae SK;Polini A;Bhise N;Hussain MA;Lee H;Dokmeci MR;Khademhosseini A
需要开发一种能够持续监测生物标志物的有效传感平台，以评估体外类器官的功能，并评估它们在延长的时间段内对药物化合物或化学物质的生物反应。在这里，报道了一种新型无标记微流体电化学（EC）生物传感器，其具有独特的内置芯片再生能力，用于以全自动方式连续测量来自类器官培养物的细胞分泌的可溶性生物标志物，而不会减弱传感器灵敏度。微流体EC生物传感器与人类肝脏芯片平台集成，通过测量分泌的生物标志物水平持续监测类器官的代谢活性长达7天，其中类器官的代谢活性被全身应用的药物改变。微流体再生EC生物传感器成功地测量了生物标志物水平的变化，并与细胞活力和酶联免疫吸附测定分析一致，验证了独特传感平台的准确性。据信，这种能够自动和连续检测可溶性生物标志物的多功能和强大的微流体EC生物传感器将在药物毒性研究或体外平台的功效评估期间广泛用于人类类器官的长期监测。
Development of an efficient sensing platform capable of continual monitoring of biomarkers is needed to assess the functionality of the in vitro organoids and to evaluate their biological responses toward pharmaceutical compounds or chemical species over extended periods of time. Here, a novel label‐free microfluidic electrochemical (EC) biosensor with a unique built‐in on‐chip regeneration capability for continual measurement of cell‐secreted soluble biomarkers from an organoid culture in a fully automated manner without attenuating the sensor sensitivity is reported. The microfluidic EC biosensors are integrated with a human liver‐on‐a‐chip platform for continual monitoring of the metabolic activity of the organoids by measuring the levels of secreted biomarkers for up to 7 d, where the metabolic activity of the organoids is altered by a systemically applied drug. The variations in the biomarker levels are successfully measured by the microfluidic regenerative EC biosensors and agree well with cellular viability and enzyme‐linked immunosorbent assay analyses, validating the accuracy of the unique sensing platform. It is believed that this versatile and robust microfluidic EC biosensor that is capable of automated and continual detection of soluble biomarkers will find widespread use for long‐term monitoring of human organoids during drug toxicity studies or efficacy assessments of in vitro platforms.