Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunsorbant Assay of Integrin Expression Level on Cell Membrane
Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunsorbant Assay of Integrin Expression Level on Cell Membrane
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肽缀合金纳米探针：细胞膜上整合素表达水平的内在纳米酶联免疫吸附测定
DOI：
10.1021/acsnano.5b04261
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发表时间：
2015-11-01
期刊：
影响因子：
17.1
通讯作者：
Gao, Xueyun
中科院分区：
文献类型：
--
作者：
Gao, Liang;Liu, Meiqing;Gao, Xueyun
准确定量细胞表面膜蛋白的表达水平对于癌症的早期诊断和有效治疗具有重要意义。我们证明了经合理设计的多肽生物偶联的金纳米颗粒作为纳米探针对人红白血病细胞系上的整合素GPIb/IIIa具有选择性标记和准确定量的能力。通过对整合素的选择性识别和标记，利用纳米探针的双光子光致发光直接观察蛋白质在细胞膜上的空间分布。更重要的是，利用纳米探针固有的类酶催化特性，无需细胞裂解和蛋白质提取过程，就可以在扩增和可靠的比色分析中定量计算整合素在人红白血病细胞上的表达水平。此外，通过相关的电感耦合等离子体质谱测量分析了金纳米颗粒与膜蛋白的相关性，验证了新分析方法的可靠性。预计这一简便而有效的策略为快速、准确和可靠地定量细胞表面感兴趣的功能膜蛋白带来了巨大的希望。
Precisely quantifying the membrane protein expression level on cell surfaces is of vital importance for early cancer diagnosis and efficient treatment. We demonstrate that gold nanoparticle bioconjugated by a rationally designed peptide as nanoprobe possesses selective labeling and accurate quantification capacity of integrin GPIlb/IIIa on the human erythroleukemia cell line. Through selective recognition and marking of integrin, two-photon photoluminescence of the nanoprobe is exploited for direct observation of protein spatial distribution on cell membrane. More importantly, utilizing intrinsic enzyme-like catalysis property of the nanoprobe, the expression level of integrin on human erythroleukemia cells can be quantitatively counted in an amplified and reliable colorimetric assay without cell lysis and protein extraction process. In addition, the analysis of the correlation between the gold nanoparticle and the membrane protein via relevant inductively coupled plasma mass spectrometry measurement verifies the reliability of the new analytical method. It is anticipated that this facile and efficient strategy holds a great promise for a rapid, precise, and reliable quantification of interested functional membrane proteins on the cell surface.