MUCIN PROFILES IN ULCERATIVE-COLITIS WITH DYSPLASIA AND CARCINOMA
MUCIN PROFILES IN ULCERATIVE-COLITIS WITH DYSPLASIA AND CARCINOMA
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DOI：
10.1111/j.1365-2559.1988.tb02057.x
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发表时间：
1988-10-01
期刊：
影响因子：
6.4
通讯作者：
BIGGART, JD
中科院分区：
文献类型：
--
作者：
ALLEN, DC;CONNOLLY, NS;BIGGART, JD
在克罗恩性结肠炎、溃疡性结肠炎伴再生、发育不良和癌以及非结肠炎腺癌中评估粘蛋白分泌。采用高铁二胺-alcian blue （HID-AB）和高碘酸硼氢化-皂化周期酸Schiff （PB-KOH-PAS）技术分别表征了磺胺酸和唾液酸以及o -酰化唾液酸。克罗恩病、静止期和活动期溃疡性结肠炎均有黏膜增生和唾液黏液蛋白分泌增多。癌性结肠炎与非典型增生结肠炎一样，远离肿瘤的炎性黏膜唾液黏液蛋白增多。他们之间或与没有癌症的结肠炎对照组之间没有统计学差异。发育不良的隐窝经常分泌硫黏液蛋白，增加的唾液黏液蛋白在叶表面或邻近发育不良的过渡性腺体中。一项比较研究的HID-AB技术给出了完全正确的单个定量评估隐窝的定性分配。常规HID-AB染色不能识别溃疡性结肠炎的发育不良。通过PB-KOH-PAS技术，结直肠腺癌与邻近粘膜相比，o -酰化唾液黏液蛋白明显减少。溃疡性结肠炎的粘膜发育不良在o -酰化唾液酸变异中显示出类似的趋势，不同于年龄和性别匹配的结肠炎对照组。PB-KOH-PAS技术可能有助于评估溃疡性结肠炎的粘蛋白分泌，作为恶性肿瘤演变的指导。
Mucin secretion was assessed in Crohn''s colitis, in ulcerative colitis with regeneration, dysplasia and carcinoma and in non-colitic adenocarcinoma. The high iron diamine-alcian blue (HID-AB) and periodate borohydride-saponification periodic acid Schiff (PB-KOH-PAS) techniques were used to demonstrate sulphomucins and sialomucins, and O-acylated sialomucins respectively. There was mucosal hyperplasia and increased sialomucin secretion in Crohn''s disease, quiescent and active ulcerative colitis. In colitis with carcinoma inflamed mucosa away from the tumour had increased sialomucins as had coitis with dysplasia. They did not differ statistically from each other or from colitic controls without cancer. Dysplastic crypts frequently secreted sulphomucins and the increased sialomucins were in transitional-like glands in the surface fronds or adjacent to the dysplasia. A comparative study of the HID-AB technique gave total correct qualitative allocation of individual quantitatively assessed crypts. Routine HID-AB staining did not aid the recognition of dysplasia in ulcerative colitis. With the PB-KOH-PAS technique colorectal adenocarcinoma showed a significant diminution in O-acylated sialomucins compared with its adjacent mucosa. Mucosal dysplasia in ulcerative colitis displayed a similar trend in O-acylated sialic acid variants, differing with respect to age-and sex-matched colitic controls. The PB-KOH-PAS technique may be of help in assessing mucin secretion in ulcerative colitis as a guide to the evolution of malignancy.