Trans-segmental imaging in the spinal cord of behaving mice.
Trans-segmental imaging in the spinal cord of behaving mice.
复制标题
行为小鼠脊髓的跨节段成像。
DOI：
10.1038/s41587-023-01700-3
复制
发表时间：
2023
影响因子：
46.9
通讯作者：
Nimmerjahn,Axel
中科院分区：
文献类型：
--
作者：
Shekhtmeyster,Pavel;Duarte,Daniela;Carey,ErinM;Ngo,Alexander;Gao,Grace;Olmstead,JackA;Nelson,NicholasA;Nimmerjahn,Axel
脊髓回路在传递疼痛中起着至关重要的作用，但在行为正常的小鼠中，脊髓节段内部和跨节段的潜在活动模式仍然难以捉摸。我们开发了一种可穿戴的宽视场显微镜，其视野为7.9 mm2，横向分辨率为~3- 4 μm，工作距离为2.7 mm，总重量<10 g，结果表明，高度局部的疼痛机械刺激会引起多个脊柱节段广泛、协调的星形胶质细胞兴奋。
Spinal cord circuits play crucial roles in transmitting pain, but the underlying activity patterns within and across spinal segments in behaving mice have remained elusive. We developed a wearable widefield macroscope with a 7.9-mm2field of view, ~3- to 4-μm lateral resolution, 2.7-mm working distance and <10-g overall weight and show that highly localized painful mechanical stimuli evoke widespread, coordinated astrocyte excitation across multiple spinal segments.