COMPARATIVE-STUDIES ON VASCULAR ENDOTHELIUM IN-VITRO .3. EFFECTS OF CYTOKINES ON THE EXPRESSION OF E-SELECTIN, ICAM-1 AND VCAM-1 BY CULTURED HUMAN ENDOTHELIAL-CELLS OBTAINED FROM DIFFERENT PASSAGES
COMPARATIVE-STUDIES ON VASCULAR ENDOTHELIUM IN-VITRO .3. EFFECTS OF CYTOKINES ON THE EXPRESSION OF E-SELECTIN, ICAM-1 AND VCAM-1 BY CULTURED HUMAN ENDOTHELIAL-CELLS OBTAINED FROM DIFFERENT PASSAGES
复制标题
DOI：
10.1159/000163938
复制
发表时间：
1995-03-01
期刊：
影响因子：
5
通讯作者：
KIRKPATRICK, CJ
中科院分区：
文献类型：
--
作者：
KLEIN, CL;BITTINGER, F;KIRKPATRICK, CJ
内皮细胞 (EC) 对促炎细胞因子非常敏感，例如白细胞介素-1 (IL-1) 和肿瘤坏死因子 α。 （TNF α）以及细菌脂多糖。这些物质刺激 EC 分泌趋化因子并增加细胞粘附分子 (CAM) 的表达，从而导致与白细胞的相互作用发生显着改变。粒细胞和单核细胞。在这些相互作用中，E-选择素、ICAM-1 和 VCAM-1 发挥着重要作用，因为它们由 EC 呈递并与白细胞膜上相应的配体相互作用。这些粘附分子已在世界范围内使用培养的 EC 进行了各种体外实验中进行了研究。这些实验中使用了不同的通道和通道的混合物，通常不考虑结果的可比性。在本研究中，研究了暴露于IL-1β和TNFα4、8和24小时后从不同传代（第1-6代）获得的培养的人脐静脉EC（HUVEC）上E-选择素、ICAM-1和VCAM-1的表达。在之前的研究中，我们已经表明，IL-1β和TNFα以类似的方式增加HUVEC和成人EC细胞质膜上E-选择素和ICAM-1的表达，这些细胞质膜来自隐静脉和股动脉。使用比较定量细胞酶免疫分析，我们发现粘附分子的表达随着传代次数的增加而显着降低。比较不同通道中 6 至 24 小时之间的不同刺激时间段，CAM 的持久性也有所下降。这些数据表明传代次数对于EC上粘附分子的表达起着重要作用。该结果对于针对 CAM 的 EC 表现进行有意义的体外比较研究规划相关。
Endothelial cells (EC) are very responsive to proinflammatory cytokines, e.g. interleukin-1 (IL-1) and tumor necrosis factor alpha. (TNF alpha), as well as to bacterial lipopolysaccharide. EC are stimulated by these substances to secrete chemotactic factors and to increase expression of cell adhesion molecules (CAM), leading to dramatically altered interactions with leukocytes, e.g. granulocytes and monocytes. In these interactions E-selectin, ICAM-1 and VCAM-1 are known to play an important role, as they are presented by the EC and interact with corresponding ligands on the white blood cell membranes. These adhesion molecules have been studied worldwide in a variety of in vitro experiments using cultured EC. Different passages and mixtures of passages have been used in these experiments, often without any regard to the comparability of the results. In this study the expression of E-selectin, ICAM-1 and VCAM-1 on cultured human umbilical vein EC (HUVEC) obtained from different passages (passages 1-6) was studied after 4, 8 and 24 h of exposure to IL-1 beta and TNF alpha. In previous studies, we have shown that IL-1 beta and TNF alpha increase the expression of E-selectin and ICAM-1 on the cytoplasmatic membranes of HUVEC and human adult EC from the saphenous vein and femoral artery in a similar fashion. Using a comparative quantitative cell enzyme immunoassay, we found that the expression of the adhesion molecules was significantly reduced with increasing passages. There was also a decreased persistence of CAM comparing different periods of stimulation between 6 and 24 h in the different passages. These data indicate that the number of passages plays an important role in the expression of adhesion molecules on EC. The results are relevant for the meaningful planning of comparative in vitro studies on EC presentation of CAM.