Exploring Properties of a Hyperthermophilic Membrane-Bound Hydrogenase at Carbon Nanotube Modified Electrodes for a Powerful H2/O2 Biofuel Cell
Exploring Properties of a Hyperthermophilic Membrane-Bound Hydrogenase at Carbon Nanotube Modified Electrodes for a Powerful H2/O2 Biofuel Cell
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DOI：
10.1002/elan.201200405
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发表时间：
2013-03-01
期刊：
影响因子：
3
通讯作者：
Lojou, Elisabeth
中科院分区：
文献类型：
--
作者：
De Poulpiquet, Anne;Ciaccafava, Alexandre;Lojou, Elisabeth
氢化酶是许多微生物氢代谢的关键酶。由于他们开发的H2氧化的高效率和特异性，他们最近被用作高效H2/O2生物燃料电池的生物催化剂。在这项工作中，我们探索的O2-，CO-和T度抗性氢化酶的新特性。研究并优化了碳纳米管修饰电极上酶的固定化，以实现直接氢催化氧化过程的长期稳定。最后讨论了共固定化氧化还原介体在提高H2/O2燃料电池性能方面的作用。
Hydrogenases are the key enzymes for hydrogen metabolism in many microorganisms. Due to the high efficiency and specificity they developed for H2 oxidation, they have been recently used as biocatalysts for an efficient H2/O2 biofuel cell. In this work we explore new properties of an O2-, CO- and T degrees-resistant hydrogenase. Enzyme immobilization on carbon nanotube modified electrodes is studied and optimized for long-term stabilization of the direct hydrogen catalytic oxidation process. The role of co-immobilized redox mediator is finally discussed in view of H2/O2 fuel cell performance enhancement.