Age-related carbonyl stress and erythrocyte membrane protein carbonylation.
Age-related carbonyl stress and erythrocyte membrane protein carbonylation.
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DOI：
10.3233/ch-2010-1355
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发表时间：
2010
影响因子：
2.1
通讯作者：
Guolin Li;Li Liu;Hui Hu;Qiong Zhao;Fuxia Xie;Keke Chen;Shenglin Liu;Yaqin Chen;Wang Shi;D. Yin
中科院分区：
文献类型：
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作者：
Guolin Li;Li Liu;Hui Hu;Qiong Zhao;Fuxia Xie;Keke Chen;Shenglin Liu;Yaqin Chen;Wang Shi;D. Yin
反应性羰基物质（RCS）已被广泛用作氧化应激的指标。然而，羰基应激与衰老过程和红细胞生化改变的关系仍知之甚少。从874名志愿者中采集新鲜血液样本，置于含有肝素钠的真空采血管中，这些志愿者根据年龄分为年轻组、成年组和老年组。采集血样后24 h内检测不同年龄组血浆RCS和巯基浓度，并检测成人组红细胞膜蛋白羰基化水平。结果表明，血浆硫醇浓度在老化过程中逐渐下降，所有三组之间的p值均小于0.05。不同年龄组血浆RCS浓度与年龄呈非线性相关。青年组略高于成年组（P <0.01），低于老年组（P < 0.01）。红细胞膜蛋白羰基化与血浆RCS浓度呈正相关（P < 0.01），而与血浆巯基浓度无相关性。我们的结论是，较高水平的RCS，而不是较低水平的硫醇，在血浆中是一个直接的危险因素，红细胞膜的蛋白质羰基化。由于衰老过程中巯基水平的降低和RCS水平的增加，从RCS相关的氧化还原别稳态向羰基应激的转变将有助于与年龄相关的生物功能障碍，甚至衰老过程。
Reactive carbonyl species (RCS) have been widely used as indicators of oxidative stress. However, the associations of carbonyl stress with aging process and biochemical alteration of erythrocyte are still poorly understood. Fresh blood samples in vacutainer tubes containing sodium heparinate were obtained from 874 volunteers who were divided into young, adult and old groups based on their age. Plasma RCS and thiols concentrations between different age groups and erythrocyte membrane protein carbonylation in the adult group were detected within 24h of the blood sampling. Results showed that the plasma thiols concentration decreased gradually during aging process, and the p-values between all three groups are less than 0.05. The plasma RCS concentration in different age groups showed a nonlinear association with age. The levels in the young group were slightly higher than the adult group (not significant) and lower than the old group (p < 0.01). The protein carbonylation of erythrocyte membrane was positively correlated with plasma RCS concentration (p < 0.01), but not plasma thiols concentration. We conclude that higher levels of RCS, not lower levels of thiols, in plasma are a direct risk factor for the protein carbonylation of erythrocyte membrane. Owing to the decrease of thiols levels and increase of RCS levels during aging process, a shift from RCS-related redox allostasis to carbonyl stress would contribute to age-related biological dysfunction and even aging process.