Potential biomarkers for esophageal carcinoma detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Potential biomarkers for esophageal carcinoma detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
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DOI：
10.1515/cclm.2010.138
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发表时间：
2010-06-01
影响因子：
6.8
通讯作者：
Wang, Shi-Jie
中科院分区：
文献类型：
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作者：
Liu, Li-Hua;Shan, Bao-En;Wang, Shi-Jie
背景：目前，没有令人满意的生物标志物可用于筛查食管鳞状细胞癌（ESCC）。采用基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption/ionization time-of-flight mass spectrometry，MALDI-TOF MS）技术，建立早期诊断食管鳞癌的血清蛋白质指纹模型。将等离子体应用于弱阳离子交换磁珠（WCX-MB）后，产生质荷比（m/z）的蛋白质组学谱。结果：利用MALDI-TOF MS建立了3种鉴别模式，模式1由11个蛋白质峰组成，将ESCC患者与健康人区分开来，敏感性为90.0%，特异性为88.4%。模式2由8个蛋白质峰组成，将I期和II期ESCC与III期和IV期ESCC分开，灵敏度为92.9%，特异性为82.3%。结论：MALDI-TOF MS结合MB分离技术检测食管鳞癌患者血清蛋白质的灵敏度和特异性均明显提高，且具有较高的特异性和敏感性。临床化学实验室医学2010;48：855-61。
Background: Currently, no satisfactory biomarkers are available to screen for esophageal squamous cell carcinoma (ESCC). The goal of this study was to find biomarkers and establish a serum protein fingerprint model for early diagnosis of ESCC using the ClinProt protocol of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).Methods: Serum samples were collected from 62 patients with ESCC, nine patients with esophageal adenocarcinoma (EA) and 38 healthy individuals. Proteomic spectra of mass to charge ratio (m/z) were generated following the application of plasma to weak cationic-exchanger magnetic beads (WCX-MB). The spectral data were analyzed using a support vector machine, and potential biomarkers were chosen for system training and used to construct diagnostic models.Results: Three differential patterns were established using MALDI-TOF MS. Pattern 1, consisting of 11 protein peaks, separated ESCC patients from the healthy individuals with a sensitivity of 90.0% and a specificity of 88.4%. Pattern 2, consisting of eight protein peaks, separated ESCC in stage I and stage II from stage III and stage IV with a sensitivity of 92.9% and a specificity of 82.3%. Pattern 3, consisting of seven protein peaks, separated ESCC from EA with a sensitivity of 91.3% and a specificity of 80.0%.Conclusions: These results suggested that MALDI-TOF MS combined with MB separation yields significantly higher sensitivity and specificity for the detection of serum protein in patients with ESCC. Clin Chem Lab Med 2010;48:855-61.