CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.
CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.
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作者：
先天性免疫细胞，如肠上皮细胞、树突状细胞（DC）、巨噬细胞、粒细胞和先天性淋巴样细胞，为肠道病原体提供了第一道防线。为了研究CX 3CR 1 + DC和巨噬细胞在宿主防御中的作用，我们用啮齿类柠檬酸杆菌感染CX 3CR 1-GFP动物。当转基因CX 3CR 1-GFP动物感染天然小鼠病原体C.在啮齿类动物中，CX 3CR 1 −/−动物表现出C.啮齿类动物与野生型B6动物相比（年龄和性别匹配）。C.啮齿类动物与白细胞介素（IL）-22表达减少有关。In C.在啮齿类感染的CX 3CR 1-GFP动物中，产生IL-22的淋巴组织诱导细胞（LTi细胞）在不存在CX 3CR 1的情况下被选择性地减少。IL-22表达降低与抗微生物肽RegIIIβ和RegIIIγ表达降低相关。在CX 3CR 1-GFP × CD11c.DOG动物中通过注射白喉毒素消耗CX 3CR 1+细胞证实了CX 3CR 1+吞噬细胞在建立IL-22产生中的作用，支持了C.啮齿类感染
Innate immune cells, such as intestinal epithelial cells, dendritic cells (DCs), macrophages, granulocytes, and innate lymphoid cells provide a first line of defence to enteric pathogens. To study the role of CX3CR1+ DCs and macrophages in host defence, we infected CX3CR1-GFP animals with Citrobacter rodentium. When transgenic CX3CR1-GFP animals are infected with the natural mouse pathogen C. rodentium, CX3CR1−/− animals showed a delayed clearance of C. rodentium as compared with (age- and sex-matched) wild-type B6 animals. The delayed clearance of C. rodentium is associated with reduced interleukin (IL)-22 expression. In C. rodentium-infected CX3CR1-GFP animals, IL-22 producing lymphoid-tissue inducer cells (LTi cells) were selectively reduced in the absence of CX3CR1. The reduced IL-22 expression correlates with decreased expression of the antimicrobial peptides RegIIIβ and RegIIIγ. The depletion of CX3CR1+ cells by diphtheria toxin injection in CX3CR1-GFP × CD11c.DOG animals confirmed the role of CX3CR1+ phagocytes in establishing IL-22 production, supporting the clearance of a C. rodentium infection.
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影响因子：
64.8
作者：
Cella, Marina;Fuchs, Anja;Vermi, William;Facchetti, Fabio;Otero, Karel;Lennerz, Jochen K. M.;Doherty, Jason M.;Mills, Jason C.;Colonna, Marco
通讯作者：
Colonna, Marco
DOI：
10.1038/jid.2009.65
发表时间：
2009-09
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Ishida, Yuko;Hayashi, Takahito;Kondo, Toshikazu
通讯作者：
Kondo, Toshikazu
影响因子：
29.4
作者：
Brand, S;Sakaguchi, T;Reinecker, HC
通讯作者：
Reinecker, HC
影响因子：
9.8
作者：
Brand, S;Hofbauer, K;Lohse, P
通讯作者：
Lohse, P