Synthesis, crystal structures, photoluminescence properties and DNA binding of triazine-nickel(II) complexes for DNA detection
Synthesis, crystal structures, photoluminescence properties and DNA binding of triazine-nickel(II) complexes for DNA detection
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DOI：
10.1016/j.saa.2015.05.063
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发表时间：
2015-12-05
影响因子：
4.4
通讯作者：
Zhou, Xiao-hua
中科院分区：
文献类型：
--
作者：
Duan, Ran-ran;Ou, Zhi-bin;Zhou, Xiao-hua
本文报道了三个新的镍(II)配合物的合成：[Ni(PzTA)(2)CO3]中心点5H(2)O(PzTA=2，4-二氨基-6-(2‘-吡嗪)-1，3，5-三嗪)in 1，[NiQ(PzTA)(H2O)(2)]Cl中心点H2O(HQ=8-羟基喹啉，PYTA=2，4-二氨基-6-(2’-吡啶)-1，3，5-三嗪)in 2，[NiQ(PzTA)(H2O)(2)]Cl中心点H2O。并用紫外可见光谱、元素分析、摩尔电导和X-射线单晶衍射法对其进行了表征。用电子光谱、DNA中的溴化乙锭置换、粘度法和循环伏安法研究了配合物与ct-DNA的结合。结果表明，三种络合物的DNA结合方式均为插层，络合物1与外界静电结合。此外，这三种配合物还表现出潜在的抗氧化活性。有趣的是，我们在荧光光谱中发现1对氧和非水溶剂的极性很敏感。在中性水溶液中，2和3的荧光较弱，但加入ct-DNA后荧光强度大大增强。因此，2和3可作为DNA荧光探针用于DNA检测，线性范围分别为0.01-20和0.02-30微克毫升，检测灵敏度分别为1.61和4.90微克毫克毫升。这些发现表明，1可能是非水无氧环境中潜在的光学探针，而2和3是以DNA为靶标的探针。(C)2015爱思唯尔B.V.保留所有权利。
We report here the synthesis of three new nickel(II) complexes: [Ni(PzTA)(2)CO3]center dot 5H(2)O (PzTA = 2,4-diamino-6-(2'-pyrazin)-1,3,5-triazine) in 1, [NiQ(PyTA)(H2O)(2)]Cl center dot H2O (HQ = 8-hydroxyquinoline, PyTA = 2,4-diamino-6-(2'-pyridyl)-1,3,5-triazine) in 2, [NiQ(PzTA)(H2O)(2)]Cl center dot H2O in 3, and they were characterized by UV spectroscopy, elemental analysis, molar conductivity and X-ray single crystal diffraction. Binding of the complexes to ct-DNA was investigated with electronic spectroscopy, ethidium bromide displacement from DNA, viscometry and cyclic voltammetry. The results depicted the DNA binding mode of the three complexes was intercalation, and complex 1 together with external static-electricity. Moreover, the three complexes also presented potential anti-oxidant activity. Interestingly, we found 1 was sensitive to oxygen and to the polarity of nonaqueous solvents in fluorescence spectroscopy. Fluorescence of 2 and 3 is weak in neutral aqueous solvents, but is greatly enhanced by addition of ct-DNA Thus, 2 and 3 can be used to DNA detection as DNA fluorescence probes with a LOD of 1.61 ng mL(-1), 4.90 ng mL(-1) for the relative wide linear range of 0.01-20 mu g mL(-1), 0.02-30 mu g mL(-1), respectively. These findings indicate that 1 may be a potential optical probe for oxygen-free environments in nonaqueous form, while 2 and 3 were DNA-targeted probes. (C) 2015 Elsevier B.V. All rights reserved.