Photochemical inactivation of viruses and bacteriophage in plasma and plasma fractions.
Photochemical inactivation of viruses and bacteriophage in plasma and plasma fractions.
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发表时间：
1992
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通讯作者：
P. Morel;L. Lin;G. Wiesehahn;L. Corash
中科院分区：
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作者：
P. Morel;L. Lin;G. Wiesehahn;L. Corash
与输血有关的病毒性疾病传播仍然是一个问题。已经开发了许多方法来去除血浆和血浆组分中的病毒病原体，包括：干热、湿热、溶剂-去污剂处理和免疫亲和纯化。虽然这些方法中的一些成功地灭活了致病性病毒，但灭活可能不完全或导致对不稳定血浆蛋白的损伤。我们开发了一种利用植物荧光素和长波长紫外光对病原性病毒进行光化学去污的方法。在本研究中，一系列的模型病毒已被添加到血浆和血浆组分，以检查光化学去污的效率和对不稳定的血浆凝血因子的影响。RNA和DNA病毒在允许保留凝血蛋白功能的条件下都被灭活。PCD技术似乎为血液制品的净化提供了一种有前途的解决方案。
Transfusion-associated transmission of viral diseases remains a problem. A number of methods have been developed to inactivate viral pathogens in plasma and plasma fractions, including: dry heating, wet heating, solvent-detergent treatment, and immunoaffinity purification. While some of these methods successfully inactivate pathogenic viruses, inactivation may be incomplete or result in damage to labile plasma proteins. We have developed a method of photochemical decontamination (PCD) using psoralens and long wavelength ultraviolet light to inactivate pathogenic viruses. In the present study, a spectrum of model viruses have been added to plasma and plasma fractions to examine the efficiency of photochemical decontamination and the effects on labile plasma coagulation factors. Both RNA and DNA viruses have been inactivated under conditions which permit preservation of coagulation protein function. PCD technology appears to offer a promising solution to decontamination of blood products.