Transformation of 2,4-dichlorophenoxyacetic acid in four different marine and estuarine sediments:: effects of sulfate, hydrogen and acetate on dehalogenation and side-chain cleavage
Transformation of 2,4-dichlorophenoxyacetic acid in four different marine and estuarine sediments:: effects of sulfate, hydrogen and acetate on dehalogenation and side-chain cleavage
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DOI：
10.1111/j.1574-6941.1999.tb00602.x
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发表时间：
1999-05-01
影响因子：
4.2
通讯作者：
Young, LY
中科院分区：
文献类型：
--
作者：
Boyle, AW;Knight, VK;Young, LY
从亚瑟杀死（纽约/MJ河口）和Paleta溪（圣地亚哥湾，加利福尼亚州）的海洋和河口沉积物开始的富集，在硫酸盐（25 mM）的存在下，将2，4-二氯苯氧乙酸转化为4-氯苯酚。在新泽西州塔克顿和亚麻塘（斯托尼布鲁克，纽约州）的另外两种海洋沉积物中未观察到2，4-二氯苯氧乙酸的转化。在亚瑟基尔和圣地亚哥富集中，2，4-二氯苯氧乙酸损失之前的滞后期或去除50% 2，4-二氯苯氧乙酸所需的时间（t（50））不受硫酸盐存在的影响，尽管在这些富集中加入氢气和乙酸盐会降低滞后期和t（50）值。2，4-二氯苯氧乙酸转化的第一步是脱侧链形成2，4-二氯苯酚，然后将其脱氯为4-氯苯酚。第二代亚瑟杀死培养物将2，4-二氯苯氧乙酸脱氯为4-氯苯氧乙酸。这种脱氯发生在硫酸盐（25 mM）的存在和不存在下，然而，硫酸盐降低脱氯率约50%。第二代培养接种硫酸盐修正富集消耗硫酸盐和脱氯2，4-二氯苯氧乙酸4-氯苯氧乙酸同时提供氢气和乙酸时，脱氯率的两倍，没有收到氢气和乙酸的文化。硫酸盐消耗仅发生在补充有氢和乙酸盐的培养物中。这些结果表明，脱氯的有机氯农药在海洋和河口沉积物中发生在硫酸盐的存在下，除了容易利用的碳和电子供体可以刺激脱氯活性。(C)1999年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Enrichments initiated with marine and estuarine sediments from the Arthur Kill (NY/MJ estuary) and Paleta Creek (San Diego Bay, CA), transformed 2,4-dichlorophenoxyacetic acid to 4-chlorophenol in the presence of sulfate (25 mM). Transformation of 2,4-dichlorophenoxyacetic acid was not observed with two other marine sediments from Tuckerton, NJ and Flax Pond (Stony Brook, NY). The lag period prior to 2,4-dichlorophenoxyacetic acid loss or the lime required for 50% removal of 2,4-dichlorophenoxyacetic acid (t(50)) in Arthur Kill and San Diego enrichments was not affected by the presence of sulfate, although the addition of hydrogen and acetate decreased the lag periods and t(50) values in these enrichments. The first step in the transformation of 2,4-dichlorophenoxyacetic acid was side-chain removal forming 2,4,dichlorophenol which was then dechlorinated to 4-chlorophenol. Second-generation Arthur Kill cultures dechlorinated 2,4-dichlorophenoxyacetic acid to 4-chlorophenoxyacetic acid. This dechlorination occurred both in the presence and absence of sulfate (25 mM); however, sulfate reduced the rate of dechlorination by approximately 50%. Second-generation cultures inoculated with the sulfate-amended enrichments consumed sulfate and dechlorinated 2,4-dichlorophenoxyacetic acid to 4-chlorophenoxyacetic acid concurrently when supplied with hydrogen and acetate; the rate of dechlorination was twice that of cultures that did not receive hydrogen and acetate. Sulfate consumption occurred only in cultures supplemented with hydrogen and acetate. These results indicate that dechlorination of an organochlorine pesticide in marine and estuarine sediments occurs in the presence of sulfate and the addition of readily utilizable carbon and electron donors can stimulate dechlorinating activity. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.