Clinical evaluation of a multiparametric ELISA as a rapid tool for routinely diagnosing IgG-mediated autoimmune blistering dermatoses in ethnic Slavs
Clinical evaluation of a multiparametric ELISA as a rapid tool for routinely diagnosing IgG-mediated autoimmune blistering dermatoses in ethnic Slavs
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DOI：
10.1002/jcla.22336
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发表时间：
2018-05-01
影响因子：
2.7
通讯作者：
Dmochowski, Marian
中科院分区：
文献类型：
--
作者：
Gornowicz-Porowska, Justyna;Seraszek-Jaros, Agnieszka;Dmochowski, Marian
背景：自身免疫性水疱性皮肤病（ABDs）诊断的技术创新旨在实现多途径诊断。两种多参数ELISA检测可用于ABD血清学。目的是比较多参数和单特异性ELISA的诊断准确性，并检查符合传统的诊断设置ABDs的诊断价值/协议的多变量ELISA。方法：总共，128例疑似ABDs患者的血清进行了研究（27血清，以比较ELISA）。多变量ELISA（检测IgG对桥粒芯糖蛋白1和3-DSG 1/3; BP 180，BP 230，envoplakin，VII型胶原），单变量ELISA，和统计分析performed.Results：与使用疑似ABDs患者的血清，多参数ELISA产生与传统的逐步诊断的84%的协议。BP 180和BP 230的多变量ELISA与单特异性ELISA相比，敏感性分别为87.5%和80%，特异性分别为87.5%和91%，可靠性分别为87.5%和80%，阴性预测值分别为87.5%和91%。与单特异性ELISA相比，DSG 1和DSG 3的多变量ELISA的敏感性分别为50%和80%，特异性分别为100%和80%，可靠性分别为85%和80%，阳性预测值分别为100%和57%，阴性预测值分别为82%和92%。BP 180和BP 230的诊断符合率高于DSG 1和DSG 3的诊断符合率。结论：多变量ELISA试验结合临床检查和DIF是诊断斯拉夫人ABD的最低限度的方法。
Background: Technical innovation of autoimmune blistering dermatoses (ABDs) diagnosis aimed at multiplex approach. Two multiparametric ELISA tests are commercially available for ABDs serology. The aim was to compare diagnostic accuracy of multiparametric and monospecific ELISAs and to examine the diagnostic value/agreement of multivariant ELISA in compliance with traditional diagnostic setup for ABDs.Methods: In total, 128 sera from suspected ABDs patients were studied (27 sera in order to compare ELISAs). Multivariant ELISA (detection of IgG against desmoglein 1 and 3-DSG1/3; BP180, BP230, envoplakin, type VII collagen), monovariant ELISA, and statistical analysis were performed.Results: With the use of sera from patients with suspected ABDs, the multiparametric ELISA yield an agreement of 84% with traditional stepwise diagnostics. Multivariant ELISA with BP180 and BP230 showed 87.5% and 80% sensitivity, 87.5% and 91% specificity, 87.5% reliability as well as 87.5% and 80% positive predictive value, 87.5% and 91% negative predictive value, respectively, in relation to monospecific ELISA. Multivariant ELISA with DSG1 and DSG3 showed 50% and 80% sensitivity, 100% and 80% specificity, 85% and 80% reliability as well as 100% and 57% positive predictive value, 82% and 92% negative predictive value, respectively, in relation to monospecific ELISA. A better rate of agreement was observed among ELISA systems with BP180 and BP230, than with ELISA systems with DSG1 and DSG3.Conclusion: Multivariant ELISA test combined with clinical examinations and DIF is recommended as a minimal approach to diagnosing ABDs in ethnic Slavs.