Inherent properties of somatic hypermutation as revealed by human non-productive VH6 immunoglobulin rearrangements.
Inherent properties of somatic hypermutation as revealed by human non-productive VH6 immunoglobulin rearrangements.
复制标题
人类非生产性 VH6 免疫球蛋白重排揭示了体细胞超突变的固有特性。
DOI：
10.1046/j.1365-2567.1998.00427.x
复制
发表时间：
1998
期刊：
影响因子：
6.4
通讯作者：
Insel,RA
中科院分区：
文献类型：
--
作者：
Varade,WS;Carnahan,JA;Kingsley,PD;Insel,RA
为了研究在不存在抗原选择的情况下人免疫球蛋白基因的体细胞超突变的固有性质，表征了通过消减杂交富集的人非生产性VH6重排的突变。分离了由9个非生产性重排产生的10个独特克隆。突变频率为3.0%。对这些突变的分析显示了转换和胞嘧啶（C）至鸟嘌呤（G）和G至C颠换的内在偏倚。突变靶向的DNA链的偏倚不明显。基于一级DNA序列，互补决定区（CDR）中的置换突变发生频率高于预期。这种将置换突变靶向至CDR可以解释灵长类动物中VH6序列的保守性。
To study inherent properties of somatic hypermutation of human immunoglobulin genes in the absence of antigen selection, mutations of human non‐productive VH6 rearrangements enriched by subtractive hybridization were characterized. Ten unique clones arising from nine non‐productive rearrangements were isolated. The frequency of mutation was 3·0%. Analysis of these mutations showed intrinsic bias for transitions and cytosine (C) to guanine (G) and G to C transversions. Bias for the strand of DNA targeted by mutation was not evident. Replacement mutations in the complementarity‐determining region (CDR) occurred more frequently than expected based on the primary DNA sequence. This targeting of replacement mutations to the CDR may explain the conservation of the VH6 sequence in primates.