Rapid and Sensitive Detection of Troponin I in Human Whole Blood Samples by Using Silver Nanoparticle Films and Microwave Heating
Rapid and Sensitive Detection of Troponin I in Human Whole Blood Samples by Using Silver Nanoparticle Films and Microwave Heating
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DOI：
10.1373/clinchem.2010.159889
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发表时间：
2011-05-01
影响因子：
9.3
通讯作者：
Grell, Tsehai A. J.
中科院分区：
文献类型：
--
作者：
Aslan, Kadir;Grell, Tsehai A. J.
背景：心血管疾病是发达国家死亡的主要原因之一。人们普遍认为，肌钙蛋白I（TnI）可用于评估的心肌infarct.METHODS：我们研究了使用的微波加速和金属增强荧光（MA-MEF），一种技术的基础上结合使用低功率微波加热，银纳米粒子薄膜（SNFs），和荧光光谱检测TnI从人全血样品。SNFs沉积到胺改性的玻璃显微镜载玻片上通过使用托伦的反应方案，其特征在于通过光学吸收光谱和扫描电子显微镜。在室温条件下，用荧光免疫分析法检测SNFs上缓冲液和人全血样品中的TnI（对照免疫测定，2 h总测定时间）或微波加热（基于MA-MEF的免疫测定，1分钟总测定时间）。我们发现，在对照免疫测定和基于MA-MEF的免疫测定中，缓冲溶液中TnI的检测下限为0.1 μ g/ml。L和0.005 μ g/L。然而，由于孵育步骤5分钟内全血凝固，我们无法在对照免疫测定中检测到全血样本中的TnI。使用的MA-MEF技术允许检测TnI从全血样品在1分钟内与0.05 μ g/L的检测下限。结论：MA-MEF为基础的免疫测定是最快的报告的定量检测方法之一检测TnI在人全血中，并具有低检测限与市售的免疫测定类似。(C)2011年美国临床化学协会
BACKGROUND: Cardiovascular diseases are among the leading causes of mortality in developed countries. It is widely recognized that troponin I (TnI) can be used for the assessment of a myocardial infarction.METHODS: We investigated the use of the microwave-accelerated and metal-enhanced fluorescence (MA-MEF), a technique based on the combined use of low-power microwave heating, silver nanoparticle films (SNFs), and fluorescence spectroscopy for the detection of TnI from human whole blood samples. SNFs were deposited onto amine-modified glass microscope slides by use of Tollen's reaction scheme and characterized by optical absorption spectroscopy and scanning electron microscopy. The detection of TnI from buffer solutions and human whole blood samples on SNFs was carried out by using fluorescence-based immunoassays at room temperature (control immunoassay, 2 h total assay time) or microwave heating (MA-MEF-based immunoassay, 1 min total assay time).RESULTS: We found that the lower limits of detection for TnI from buffer solutions in the control immunoassay and MA-MEF-based immunoassay were 0.1 mu g/L and 0.005 mu g/L, respectively. However, we were unable to detect TnI in whole blood samples in the control immunoassays owing to the coagulation of whole blood within 5 min of the incubation step. The use of the MA-MEF technique allowed detection of TnI from whole blood samples in 1 min with a lower detection limit of 0.05 mu g/L.CONCLUSIONS: The MA-MEF-based immunoassay is one of the fastest reported quantitative detection methodos for detection of TnI in human whole blood and has low detection limits similar to those obtained with commercially available immunoassays. (C) 2011 American Association for Clinical Chemistry