Direct reconstruction in CT-analogous pharmacokinetic diffuse fluorescence tomography: two-dimensional simulative and experimental validations
Direct reconstruction in CT-analogous pharmacokinetic diffuse fluorescence tomography: two-dimensional simulative and experimental validations
复制标题
CT 类似药代动力学漫射荧光断层扫描的直接重建：二维模拟和实验验证
DOI：
10.1117/1.jbo.21.4.046007
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发表时间：
2016
影响因子：
3.5
通讯作者：
Feng Gao
中科院分区：
文献类型：
--
作者：
Xin Wang;Yanqi Zhang;Limin Zhang;Jiao Li;Zhongxing Zhou;Huijuan Zhao;Feng Gao
摘要。我们提出了一种基于ct类似扫描模式的药物动力学漫射荧光断层扫描（DFT）直接重建的通用策略，该策略通过将区室动力学模型纳入自适应扩展卡尔曼滤波方案并使用瞬时采样数据集，可以实现体内小动物体内吲啶绿药动率图像的一步重建。与已有的间接和直接方法相比，该方法消除了DFT反演的中间误差，降低了仪器获取完整360度投影的高时间分辨率数据集的昂贵要求。通过双室模型的二维模拟和单室模型的中导模拟实验验证了该方法的有效性，表明该方法可以同时估计室浓度和药代动力学速率，具有良好的定量和定位精度，非常适合基于高灵敏度光子计数技术的高成本效益和密集采样仪器。
Abstract. We present a generalized strategy for direct reconstruction in pharmacokinetic diffuse fluorescence tomography (DFT) with CT-analogous scanning mode, which can accomplish one-step reconstruction of the indocyanine-green pharmacokinetic-rate images within in vivo small animals by incorporating the compartmental kinetic model into an adaptive extended Kalman filtering scheme and using an instantaneous sampling dataset. This scheme, compared with the established indirect and direct methods, eliminates the interim error of the DFT inversion and relaxes the expensive requirement of the instrument for obtaining highly time-resolved date-sets of complete 360 deg projections. The scheme is validated by two-dimensional simulations for the two-compartment model and pilot phantom experiments for the one-compartment model, suggesting that the proposed method can estimate the compartmental concentrations and the pharmacokinetic-rates simultaneously with a fair quantitative and localization accuracy, and is well suitable for cost-effective and dense-sampling instrumentation based on the highly-sensitive photon counting technique.