Age-related gene expression profiles of rhesus monkey bone marrow-derived mesenchymal stem cells
Age-related gene expression profiles of rhesus monkey bone marrow-derived mesenchymal stem cells
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DOI：
10.1002/jcb.21498
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发表时间：
2008-03-01
影响因子：
4
通讯作者：
Tarantal, Alice F.
中科院分区：
文献类型：
--
作者：
Hacia, Joseph G.;Lee, C. Chang I.;Tarantal, Alice F.
本研究的目的是阐明恒河猴骨髓间充质干细胞（rhMSC）的基因表达谱的年龄相关差异，从胎儿，婴儿和成人供体获得相关的生长和其他属性。虽然在rhMSC基因表达谱中观察到高度的相似性时，比较三个年龄组，显着差异被发现，强烈平行的人MSC的基因表达谱。在一般情况下，有一个趋势，随着供体年龄的增加与分化和生长停滞的转录本丰度增加。相反，参与RNA加工和基因表达负调控的转录物随着供体年龄的增加而呈下降趋势。总的来说，发现观察到的基因表达谱与来自老年个体的MSC显示增殖能力降低的观察结果相似。这些数据突出了使用非人灵长类动物研究干细胞和祖细胞特性以及未来治疗的重要性。
The objective of this study was to elucidate age-related differences in gene expression profiles of rhesus monkey bone marrow-derived mesenchymal stem cells (rhMSC) obtained from fetal, infant, and adult donors relevant to their growth and other properties. Although a high degree of similarity was observed in the rhMSC gene expression profiles when comparing the three age groups, significant differences were found that strongly parallel gene expression profiles of human MSC. In general, there was a trend towards increased abundance of transcripts associated with differentiation and growth arrest with increasing donor age. Conversely, transcripts involved in RNA processing and the negative regulation of gene expression showed a downward trend with increasing donor age. Overall, the observed gene expression profiles were found to be similar to observations that MSC from older individuals show diminished proliferative capacity. These data highlight the importance of use of non-human primates to study the properties of stem and progenitor cells, and for future therapies.