Cutaneous Lymphoma at Injection Sites: Pathological, Immunophenotypical, and Molecular Characterization in 17 Cats
Cutaneous Lymphoma at Injection Sites: Pathological, Immunophenotypical, and Molecular Characterization in 17 Cats
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DOI：
10.1177/0300985815623620
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发表时间：
2016-07-01
影响因子：
2.4
通讯作者：
Affolter, V. K.
中科院分区：
文献类型：
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作者：
Roccabianca, P.;Avallone, G.;Affolter, V. K.
猫原发性皮肤淋巴瘤（FPCLs）占猫所有淋巴瘤的0.2%至3%，并且更常见的是真皮非上皮性小t细胞肿瘤。FPCL的出现似乎与猫白血病病毒（FeLV）血清学阳性或皮肤炎症无关。从47例fcls中选择17例肿瘤发生部位有疫苗注射史的皮肤淋巴瘤。评估临床表现、组织学、免疫表型、FeLV p27和gp70表达及克隆性。大多数为公猫（12/17），家养短毛猫（13/17），平均年龄11.3岁。5只猫注射后的发育时间从15天到大约9年不等。在诊断时，17只猫中有11只没有内部疾病的证据。淋巴瘤发生在肩胛间（8/17）、胸部（8/17）和侧腹（1/17）皮肤区域；缺乏epitheliotropism;表现为坏死（16/17）、血管中心性（13/17）、血管侵犯（9/17）、血管破坏（8/17）和外周血淋巴细胞聚集性炎症（14/17）。FeLV gp70和/或p27蛋白在17个肿瘤中的10个中表达。根据世界卫生组织的分类、免疫表型和克隆性，将病变分为大b细胞淋巴瘤（11/17）、间变性大t细胞淋巴瘤（3/17）、自然杀伤细胞样淋巴瘤（1/17）和外周t细胞淋巴瘤（1/17）。1例血统不确定。注射部位皮肤淋巴瘤（CLIS）与猫注射部位肉瘤和人类亚急性至慢性炎症中发生的淋巴瘤有一些共同的临床和病理特征。由注射和FeLV表达的再激活引起的持续炎症可能促成了CLIS的出现。
Feline primary cutaneous lymphomas (FPCLs) account for 0.2% to 3% of all lymphomas in cats and are more frequently dermal nonepitheliotropic small T-cell tumors. Emergence of FPCL seems unrelated to feline leukemia virus (FeLV) serological positivity or to skin inflammation. A total of 17 cutaneous lymphomas with a history of vaccine injection at the site of tumor development were selected from 47 FPCLs. Clinical presentation, histology, immunophenotype, FeLV p27 and gp70 expression, and clonality were assessed. A majority of male (12/17), domestic short-haired (13/17) cats with a mean age of 11.3 years was reported. Postinjection time of development ranged from 15 days to approximately 9 years in 5 cats. At diagnosis, 11 of 17 cats had no evidence of internal disease. Lymphomas developed in interscapular (8/17), thoracic (8/17), and flank (1/17) cutaneous regions; lacked epitheliotropism; and were characterized by necrosis (16/17), angiocentricity (13/17), angioinvasion (9/17), angiodestruction (8/17), and peripheral inflammation composed of lymphoid aggregates (14/17). FeLV gp70 and/or p27 proteins were expressed in 10 of 17 tumors. By means of World Health Organization classification, immunophenotype, and clonality, the lesions were categorized as large B-cell lymphoma (11/17), anaplastic large T-cell lymphoma (3/17), natural killer cell-like (1/17) lymphoma, or peripheral T-cell lymphoma (1/17). Lineage remained uncertain in 1 case. Cutaneous lymphomas at injection sites (CLIS) shared some clinical and pathological features with feline injection site sarcomas and with lymphomas developing in the setting of subacute to chronic inflammation reported in human beings. Persistent inflammation induced by the injection and by reactivation of FeLV expression may have contributed to emergence of CLIS.