Growth hormone secretion in the rat: metabolic clearance and secretion rates.
Growth hormone secretion in the rat: metabolic clearance and secretion rates.
复制标题
大鼠生长激素分泌：代谢清除率和分泌率。
DOI：
10.1210/endo-86-2-305
复制
发表时间：
1970
期刊：
影响因子：
4.8
通讯作者：
L. Bernardis
中科院分区：
文献类型：
--
作者：
L. Frohman;L. Bernardis
用131I-大鼠生长激素持续输注和单次注射131I-生长激素和未标记的垂体生长激素测定了麻醉成年大鼠生长激素的代谢清除和分泌速率。3种方法测得的代谢清除率相近，平均为1.19ml/min。恒定输液法测定的平均基础分泌率为13.1 ng/min。在接受下丘脑腹内侧刺激的大鼠中，血浆生长激素水平在10分钟内上升了5倍，但与受刺激的大脑皮层中的大鼠相比，没有发现垂体生长激素的枯竭，在大脑皮层中，血浆生长激素没有上升。根据血浆生长激素的变化和代谢清除率的计算表明，下丘脑刺激所分泌的生长激素不到垂体生长激素含量的1%，从而解释了观察到的垂体生长激素含量的缺乏。
The metabolic clearance and secretion rates of growth hormone have been determined in anesthetized adult rats using a constant infusion of 131I-rat growth hormone and single injections of both 131I-growth hormone and unlabeled pituitary growth hormone. Similar values were obtained for the metabolic clearance rate using the 3 techniques, with a mean of 1.19 ml/min. The mean basal secretion rate determined by the constant infusion technique was 13.1 ng/min. In rats subjected to ventromedial hypothalamic stimulation, plasma growth hormone levels rose 5-fold within 10 min, but no depletion of pituitary growth hormone was noted as compared to rats stimulated in the cerebral cortex where plasma growth hormone did not rise. Calculations using the changes in plasma growth hormone and the metabolic clearance rate indicated that the quantity of growth hormone secreted in response to hypothalamic stimulation was less than 1 % of the pituitary growth hormone content, thereby explaining the lack of observed pituitary ...