In vivo estimation of murine iris stiffness using finite element modeling.
In vivo estimation of murine iris stiffness using finite element modeling.
复制标题
使用有限元建模体内估计小鼠虹膜硬度。
DOI：
10.1016/j.exer.2020.108374
复制
发表时间：
2021-01
影响因子：
3.4
通讯作者：
Ethier CR
中科院分区：
文献类型：
--
作者：
Lee C;Li G;Stamer WD;Ethier CR
虹膜在某些类型的青光眼中起重要作用，包括原发性闭角型青光眼和色素性青光眼。虹膜力学在一些动物物种中响应于眼内压变化而影响小梁网变形方面也很重要。尽管小鼠被广泛用于研究眼部疾病，包括青光眼，但小鼠虹膜的体内生物力学特性尚不清楚。因此，本研究的主要目的是估计小鼠虹膜的生物力学刚度。我们使用了活体小鼠（n = 13，年龄= 7.3 ± 3.2 [平均值±标准差]个月）眼前节的光学相干断层扫描（OCT）图像，在连续增加的IOP水平下观察IOP依赖性虹膜变形。然后，我们使用逆有限元模型来预测虹膜变形在相同的条件下，通过最大限度地提高OCT数据和数值模拟之间的一致性来估计虹膜刚度。我们的结果显示，体内小鼠虹膜硬度为96.1 ± 54.7 kPa（平均值± SD），与年龄无关，但取决于性别。我们的结果进一步显示了反向瞳孔阻滞的强有力证据，即使前房压设置为更高水平，平均后房压仍保持在约12 mmHg。我们的方法来监测虹膜刚度在体内是适用于研究潜在的变化虹膜刚度在各种病理生理条件下，因此具有显着的潜力，涉及虹膜生物力学的眼部疾病的临床护理。
The iris plays an important role in certain types of glaucoma, including primary angle-closure glaucoma and pigmentary glaucoma. Iris mechanics are also important in influencing trabecular meshwork deformation in response to intraocular pressure changes in some animal species. Although mice are widely used to study ocular disease, including glaucoma, the in vivo biomechanical properties of the murine iris are unknown. Thus, the primary objective of this study was to estimate murine iris biomechanical stiffness. We used optical coherence tomography (OCT) images of the anterior segment of living mice (n=13, age = 7.3 ± 3.2 [mean ± SD] months) at sequentially increasing IOP levels, observing IOP-dependent iris deformations. We then used an inverse finite element model to predict iris deformations under the same conditions, estimating iris stiffness by maximizing agreement between OCT data and numerical simulations. Our results show an in vivo murine iris stiffness of 96.1 ± 54.7 kPa (mean ± SD), which did not correlate with age but was dependent on gender. Our results further showed strong evidence of reverse pupillary block, with mean posterior chamber pressure remaining at approximately 12 mmHg even as anterior chamber pressure was set to much higher levels. Our approach to monitoring iris stiffness in vivo is applicable to study potential changes of iris stiffness in various pathophysiological conditions and thus has significant potential for clinical care of ocular disease involving iris biomechanics.
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DOI：
10.1096/fj.202000702rr
发表时间：
2020-08
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Li G;Schmitt H;Johnson WM;Lee C;Navarro I;Cui J;Fleming T;Gomez-Caraballo M;Elliott MH;Sherwood JM;Hauser MA;Farsiu S;Ethier CR;Stamer WD
通讯作者：
Stamer WD
影响因子：
2.4
作者：
Heys, J;Barocas, VH
通讯作者：
Barocas, VH
DOI：
10.1115/1.4001256
发表时间：
2010-07-01
影响因子：
1.7
作者：
Amini, Rouzbeh;Barocas, Victor H.
通讯作者：
Barocas, Victor H.
影响因子：
3.8
作者：
Ortiz, Sergio;Siedlecki, Damian;Marcos, Susana
通讯作者：
Marcos, Susana
影响因子：
4.4
作者：
Amini, Rouzbeh;Barocas, Victor H.
通讯作者：
Barocas, Victor H.