SMALL GTP-BINDING PROTEIN ASSOCIATED WITH GOLGI CISTERNAE
SMALL GTP-BINDING PROTEIN ASSOCIATED WITH GOLGI CISTERNAE
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DOI：
10.1038/345553a0
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发表时间：
1990-06-07
期刊：
影响因子：
64.8
通讯作者：
SARASTE, J
中科院分区：
文献类型：
--
作者：
GOUD, B;ZAHRAOUI, A;SARASTE, J
真核细胞似乎利用GTP水解来调节胞吐和胞吞作用中的囊泡运输。这方面的最佳证据来自于对酵母Saccharomyces gallae的研究，这些研究已经确定了两种小的Ras相关的GTP结合蛋白，Sec 4p和Yptlp，它们控制着分泌途径的不同阶段1 -6。在哺乳动物细胞中，不可水解的GTP类似物GTP-γS对不同转运事件的影响表明，它们也具有与酵母Sec 4p和Yptlp 7 -10功能相关的蛋白质。最近已经克隆和测序了大鼠和人的Therabgenes，并且它们的蛋白质具有与Sec 4p和Yptlp共同的高度保守的结构域（包括推定的效应子结合位点）。因此，它们是参与哺乳动物细胞内转运的GTP结合蛋白的良好候选物11，12。Rab蛋白之一（Rab 1 p）是Ypt 1 p的哺乳动物对应物（参考文献13）。在这里，我们报告的本地化的蛋白Rab 6p的高尔基体在几种细胞类型。通过免疫标记和电子显微镜观察，Rab 6p似乎主要集中在内侧和跨脑池，并分布在其整个表面。
EUKARYOTIC cells seem to use GTP hydrolysis to regulate vesicular traffic in exocytosis and endocytosis. The best evidence for this comes from studies on the yeastSaccharomyces cerevisiaethat have identified two small Ras-related GTP-binding proteins, Sec4p and Yptlp, which control distinct stages of the secretory pathway1–6. In mammalian cells the effects of a non-hydrolysable GTP analogue, GTP-γS, on different transport events have suggested that they also have proteins functionally related to yeast Sec4p and Yptlp7–10. Therabgenes have recently been cloned and sequenced for rat and human and their proteins have highly conserved domains in common with Sec4p and Yptlp (including a putative effector binding site). They are therefore good candidates for GTP-binding proteins involved in intracellular transport in mammalian cells11,12. One of the Rab proteins (Rab1p) is the mammalian counterpart of Ypt1p (ref. 13). Here we report the localization of the protein Rab6p to the Golgi apparatus in several cell types. By immunolabelling and electron microscopy, Rab6p appears to be concentrated predominantly on the medial andtranscisternae and distributed over their entire surface.