Hypoxia-induced changes in the expression of VEGF, HIF-1 alpha and cell cycle-related molecules in ovarian cancer cells.
Hypoxia-induced changes in the expression of VEGF, HIF-1 alpha and cell cycle-related molecules in ovarian cancer cells.
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发表时间：
2002-09
影响因子：
2
通讯作者：
A. Horiuchi;T. Imai;M. Shimizu;Kenji Oka;Cuiju Wang;T. Nikaido;I. Konishi
中科院分区：
文献类型：
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作者：
A. Horiuchi;T. Imai;M. Shimizu;Kenji Oka;Cuiju Wang;T. Nikaido;I. Konishi
背景缺氧在癌症进展中以及在癌细胞从原发病灶脱离的阶段是重要的。本研究旨在探讨低氧对卵巢癌血管生成和细胞增殖的影响。材料与方法首先采用免疫组化法检测42例卵巢癌组织中VEGF的表达，并与p53表达进行相关性分析。检测4株不同p53基因状态的卵巢癌细胞系VEGF、HIF-1 α的表达、细胞周期相关分子和细胞数。结果免疫组化显示VEGF与p53表达无明显相关性。此外，缺氧通过上调HIF-1 α而增加VEGF的表达，而与p53基因状态无关。缺氧不影响细胞数量，但影响细胞周期相关分子的表达（p27增加，cyclin D1和Rb减少）。结论缺氧可增加卵巢癌细胞VEGF的表达，与p53基因状态无关。
BACKGROUND Hypoxia is important in cancer progression, and at the stage of detachment of the cancer cells from the primary lesion. This study was undertaken to analyze the effect of hypoxia on angiogenesis and cell proliferation in ovarian cancer. MATERIALS AND METHODS We first used immunohistochemistry to examine the expression of VEGF in 42 cases of ovarian carcinoma, with relevance to the p53 expression. Then, the expression of VEGF, HIF-1 alpha, cell cycle-related molecules and cell numbers were examined in 4 ovarian cancer cell lines with various p53 gene status. RESULTS Immunohistochemistry showed that there was no significant correlation between VEGF and p53 expression. Moreover, hypoxia increased the expression of VEGF via up-regulation of HIF-1 alpha irrespective of p53 gene status. However hypoxia did not change the cell numbers, but influenced the expression of cell cycle-related molecules (increased p27 and decreased cyclin D1 and Rb). CONCLUSION Hypoxia increased VEGF expression in ovarian cancer cells irrespective of p53 gene status.