Amino Acid Concentrations in HIV-Infected Youth Compared to Healthy Controls and Associations with CD4 Counts and Inflammation.
Amino Acid Concentrations in HIV-Infected Youth Compared to Healthy Controls and Associations with CD4 Counts and Inflammation.
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DOI：
10.1089/aid.2015.0369
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发表时间：
2017-07
影响因子：
1.5
通讯作者：
T. Ziegler;S. Judd;Joshua H. Ruff;G. McComsey;A. Eckard
中科院分区：
文献类型：
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作者：
T. Ziegler;S. Judd;Joshua H. Ruff;G. McComsey;A. Eckard
氨基酸在新陈代谢、细胞功能、身体组成和免疫中起着关键作用，但有关艾滋病毒患者血浆氨基酸浓度的数据很少。我们评估了HIV感染青年的血浆氨基酸浓度及其与CD4计数和炎性生物标记物的关系。将当前≥+T细胞计数高(CD500细胞/毫米~3)和低(CD_4~+500细胞/毫米~3)的HIV感染者相互比较，并与匹配的健康对照组进行比较。用氨基酸分析仪测定血浆中19种氨基酸的浓度。同时检测血浆白介素6、肿瘤坏死因子受体-I和可溶性血管细胞黏附分子-I水平。79名HIV感染者(分别有40名和39名CD4+T细胞计数较高和较低的患者)和40名对照纳入研究。CD4+T细胞计数高或低的HIV感染者之间的氨基酸浓度没有差异。当组合在一起时，HIV感染者组的血浆总氨基酸、必需氨基酸、支链氨基酸和硫磺氨基酸以及12种单独氨基酸的血浆浓度中值明显低于对照组。谷氨酸是HIV感染者组中唯一含量较高的氨基酸。无论是HIV感染组还是对照组，氨基酸终点和炎症生物标记物之间都没有显著的相关性。无论免疫状态如何，感染艾滋病毒的年轻人的血浆氨基酸浓度都低于健康对照组，而谷氨酸浓度则升高。这些发现可以为未来旨在改善受氨基酸营养影响的代谢和临床参数的干预性研究提供参考。
Amino acids play critical roles in metabolism, cell function, body composition and immunity, but little data on plasma amino acid concentrations in HIV are available. We evaluated plasma amino acid concentrations and associations with CD4 counts and inflammatory biomarkers in HIV-infected youth. HIV-infected subjects with a high (≥500 cells/mm3) and low (<500 cells/mm3) current CD4+ T cell counts were compared to one another and to a matched healthy control group. Plasma concentrations of 19 amino acids were determined with an amino acid analyzer. Plasma levels of interleukin-6, tumor necrosis factor receptor-I, and soluble vascular cellular adhesion molecule-I were also measured. Seventy-nine HIV-infected subjects (40 and 39 with high and low CD4+ T cell counts, respectively) and 40 controls were included. There were no differences in amino acid concentrations between HIV-infected subjects with high or low CD4+ T cell counts. When combined, the HIV-infected group exhibited significantly lower median plasma concentrations compared to controls for total, essential, branched-chain and sulfur amino acids, as well as for 12 individual amino acids. Glutamate was the only amino acid that was higher in the HIV-infected group. There were no significant correlations between amino acid endpoints and inflammatory biomarkers for either HIV-infected group or controls. Plasma amino acid concentrations were lower in HIV-infected youth compared to healthy controls, regardless of immune status, while glutamate concentrations were elevated. These findings can inform future interventional studies designed to improve metabolic and clinical parameters influenced by amino acid nutriture.