Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses
Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses
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DOI：
10.1016/j.gene.2003.12.024
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发表时间：
2004-03-31
期刊：
影响因子：
3.5
通讯作者：
Sekiguchia, J
中科院分区：
文献类型：
--
作者：
Serizawa, M;Yamamoto, H;Sekiguchia, J
The SigD-regulated genes in Bacillus subtilis were systematically analyzed by comparing the pattern of transcripts derived from wild-type cells with those from sigD mutant cells using DNA microarray technology. One hundred and fifty-eight genes were found to be SigD-dependent candidates, 46 of which being known SigD-regulated genes. Northern blot analysis revealed that 18 of the remaining genes were SigD-dependent. The SigD consensus sequence was newly identified in the upstream regions of nine operons (11 genes): ybdO, yfmT-yfmS, hemAT, yjcP-yjcQ, yifB, ylqB, yoaH, yscB and yxkC, and the other seven genes were assumed to be indirectly affected by a SigD mutation. Furthermore, yviE-yviF are likely to be SigD-dependent genes, because three independent sets of array data for yviE and yviF indicated they are SigD-dependent, and these genes are neighbors of flgL and hag transcribed by SigD RNA polymerase. (C) 2004 Elsevier B.V All rights reserved.
