Immobilization of chitosan film containing semaphorin 3A onto a microarc oxidized titanium implant surface via silane reaction to improve MG63 osteogenic differentiation.
Immobilization of chitosan film containing semaphorin 3A onto a microarc oxidized titanium implant surface via silane reaction to improve MG63 osteogenic differentiation.
复制标题
通过硅烷反应将含有信号蛋白3A的壳聚糖膜固定到微弧氧化钛种植体表面以改善MG63成骨分化
DOI：
10.2147/ijn.s68895
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发表时间：
2014
影响因子：
8
通讯作者：
Song Y
中科院分区：
文献类型：
--
作者：
Fang K;Song W;Wang L;Jia S;Wei H;Ren S;Xu X;Song Y
改善广泛使用的钛种植体的骨结合仍然是种植学的一个主要主题。最近，由于生物分子直接参与种植体周围的骨整合过程，在钛表面接枝生物分子引起了更多的关注。信号素3A(Semaphorin 3A，Sema3A)是一种新近被证实的骨保护分子，被认为是一种很有前途的骨疾病治疗剂，但如何将其固定在钛表面以获得长期疗效尚不清楚。在我们的研究中，我们尝试用壳聚糖包覆SeMA3A(CS/SEMA)，并通过硅烷戊二醛偶联的方式将其连接到微弧氧化的钛表面。微弧氧化可以在钛表面形成多孔形貌，共价结合涂层均匀地覆盖在孔间脊上，对原始形貌没有明显影响。在磷酸盐缓冲盐水中的最初几天中观察到Sema3A的突然释放，并可维持2周。在含有溶菌酶的磷酸盐缓冲液中包衣相似，但释放速度快得多。涂层对细胞粘附力、活性或细胞骨架排列没有显著影响，但成骨相关基因的表达显著增加，钙沉积也大量检测到。综上所述，CS/Sema的共价结合能显著促进成骨细胞的成骨分化，有望应用于钛种植体表面的生物功能化。
Improving osseointegration of extensively used titanium (Ti) implants still remains a main theme in implantology. Recently, grafting biomolecules onto a Ti surface has attracted more attention due to their direct participation in the osseointegration process around the implant. Semaphorin 3A (Sema3A) is a new proven osteoprotection molecule and is considered to be a promising therapeutic agent in bone diseases, but how to immobilize the protein onto a Ti surface to acquire a long-term effect is poorly defined. In our study, we tried to use chitosan to wrap Sema3A (CS/Sema) and connect to the microarc oxidized Ti surface via silane glutaraldehyde coupling. The microarc oxidization could formulate porous topography on a Ti surface, and the covalently bonded coating was homogeneously covered on the ridges between the pores without significant influence on the original topography. A burst release of Sema3A was observed in the first few days in phosphate-buffered saline and could be maintained for >2 weeks. Coating in phosphate-buffered saline containing lysozyme was similar, but the release rate was much more rapid. The coating did not significantly affect cellular adhesion, viability, or cytoskeleton arrangement, but the osteogenic-related gene expression was dramatically increased and calcium deposition was also abundantly detected. In conclusion, covalent bonding of CS/Sema could strongly improve osteogenic differentiation of osteoblasts and might be applied for Ti implant surface biofunctionalization.