Chromosome-wide analysis of protein binding and modifications.
Chromosome-wide analysis of protein binding and modifications.
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蛋白质结合和修饰的染色体范围分析。
DOI：
10.1007/978-1-60327-378-7_14
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Park-Sarge,Ok-Kyong
中科院分区：
文献类型：
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作者：
Sarge,KevinD;Xing,Hongyan;Park-Sarge,Ok-Kyong
为了充分了解dna结合蛋白的功能，有必要确定其在染色体中的所有结合位点，并评估每个位点在该因子整体生物学功能中的作用。将染色质免疫沉淀技术与染色体DNA微阵列分析相结合的ChIP-on-Chip方法已被证明是染色体范围内蛋白质结合位点鉴定的有力手段。这种方法也可用于描述翻译后蛋白质修饰模式的染色体范围变化，例如组蛋白修饰。本章介绍了ChIP-on-Chip分析的方法，以鉴定有丝分裂细胞中tata结合蛋白的染色体范围结合位点为例。
In order to fully understand the functions of a DNA-binding protein it is necessary to identify all of its binding sites in chromosomes and assess the role of each site in the overall biological function of the factor. An approach ChIP-on-Chip which combines the chromatin immunoprecipitation technique with chromosomal DNA microarray analysis, has proven to be a powerful means for the chromosome-wide identification of protein binding sites. This approach can also be used to characterize chromosome-wide variations in patterns of post-translational protein modifications, for example histone modifications. This chapter presents methodologies for the ChIP-on-Chip analysis, using as an example the identification of chromosome-wide binding sites for the TATA-binding protein in mitotic cells.