Increased dimeric IgA producing B cells in the bone marrow in IgA nephropathy determined by in situ hybridisation for J chain mRNA
Increased dimeric IgA producing B cells in the bone marrow in IgA nephropathy determined by in situ hybridisation for J chain mRNA
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DOI：
10.1136/jcp.49.1.38
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发表时间：
1996-01-01
影响因子：
3.4
通讯作者：
Feehally, J
中科院分区：
文献类型：
--
作者：
Harper, SJ;Allen, AC;Feehally, J
目的——探讨全身IgA免疫系统在IgA肾病发病中的可能作用。方法——研究骨髓IgA细胞中J链mRNA的表达。通过 (1) 非同位素杂交 (ISH) 和 (2) 联合免疫荧光和非同位素 ISH 检测 7 名 IgA 肾病患者和 7 名匹配对照者的骨髓环钻活检标本，以鉴定浆细胞类型。还使用标准高压液相色谱和夹心酶联免疫吸附测定法测定了血清聚合 IgA。 结果 - 非同位素 ISH 显示患者活检标本中 J 链 mRNA 阳性细胞/单位长度的数量相似（16.5 +/- 2.7 细胞/mm）和对照（17.7 +/- 2.4 细胞/mm）。联合免疫荧光和 ISH 显示，与对照组 (3 +/- 0.8%) 相比，患者中 J 链 mRNA 阳性 IgA 细胞的比例更高 (7.6 +/- 1.45%)。患者 (91 +/- 22 mg/l) 和对照组 (77 +/- 24 mg/l) 的血清聚合 IgA 相似。结论 - 这些数据表明 IgA 肾病的骨髓中出现二聚 IgA 的过量产生。
Aim-To investigate the possible role of the systemic IgA immune system in the pathogenesis of IgA nephropathy.Methods-J chain mRNA expression in the IgA cells of the bone marrow was studied. Bone marrow trephine biopsy specimens from seven patients with IgA nephropathy and seven matched controls were examined by (1) non-isotopic in hybridisation (ISH) and (2) combined immunofluorescence and non-isotopic ISH to identify the plasma cell type. Serum polymeric IgA was also determined using standard high pressure Liquid chromatography and sandwich enzyme linked immunosorbent assay.Results-Non-isotopic ISH revealed a similar number of J chain mRNA positive cells/unit length in biopsy specimens from patients (16.5 +/- 2.7 cells/mm) and controls (17.7 +/- 2.4 cells/mm). Combined immunofluorescence and ISH revealed a greater proportion of J chain mRNA positive IgA cells in patients (7.6 +/- 1.45%) compared with controls (3 +/- 0.8%). Serum polymeric IgA was similar in both patients (91 +/- 22 mg/l) and controls (77 +/- 24 mg/l).Conclusion-These data suggest that excess production of dimeric IgA occurs in the bone marrow in IgA nephropathy.