N-3-Oxo-Decanoyl-L-Homoserine-Lactone Activates Auxin-Induced Adventitious Root Formation via Hydrogen Peroxide- and Nitric Oxide-Dependent Cyclic GMP Signaling in Mung Bean
N-3-Oxo-Decanoyl-L-Homoserine-Lactone Activates Auxin-Induced Adventitious Root Formation via Hydrogen Peroxide- and Nitric Oxide-Dependent Cyclic GMP Signaling in Mung Bean
复制标题
N-3-氧代-癸酰基-L-高丝氨酸-内酯通过绿豆中过氧化氢和一氧化氮依赖性环状 GMP 信号激活生长素诱导的不定根形成
DOI：
10.1104/pp.111.185769
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发表时间：
2012-02-01
期刊：
影响因子：
7.4
通讯作者：
Hu, Xiangyang
中科院分区：
文献类型：
--
作者：
Bai, Xuegui;Todd, Christopher D.;Hu, Xiangyang
N-酰基高丝氨酸内酯（AHLs）是细菌群体感应信号分子，调节种群密度。最近的证据表明它们在植物防御反应和根系发育中的作用。过氧化氢（H2 O2）、一氧化氮（NO）和环鸟苷酸（cGMP）是参与植物各种生理过程的重要信使，但这些信使如何调节植物对N-酰基高丝氨酸-内酯信号的反应仍知之甚少。在这里，我们表明，N-3-氧代-癸酰基-高丝氨酸-内酯（3-O-C 10-HL），在其类似物与一个未取代的分支链在C3位置，有效地刺激不定根的形成和生长素反应基因的表达绿豆（Vigna radiata）幼苗外植体。这种反应是模仿外源性应用的生长素，H2 O2，NO，或cGMP同系物，但抑制与清除剂或抑制剂的H2 O2，NO，或cGMP代谢的治疗。3-O-C10-HL处理增强了生长素的向基运输，这种作用可以被H2 O2或NO清除剂逆转，但不能被cGMP合成抑制剂逆转。抑制3-O-C10-HL诱导的H2 O2或NO积累会损害生长素或3-O-C10-HL诱导的cGMP合成;然而，阻断cGMP合成并不影响生长素或3-O-C10-HL诱导的H2 O2或NO生成。此外，cGMP部分解除了H2 O2或NO清除剂对3-OC 10-HL诱导的不定根发育和生长素反应基因表达的抑制作用。这些结果表明，3-O-C10-HL，不像它的类似物与未修饰的分支链在C3的位置，可以加速生长素依赖的不定根的形成，可能通过H2 O2-和NO-依赖的cGMP信号在绿豆幼苗。
N-Acyl-homoserine-lactones (AHLs) are bacterial quorum-sensing signaling molecules that regulate population density. Recent evidence demonstrates their roles in plant defense responses and root development. Hydrogen peroxide (H2O2), nitric oxide (NO), and cyclic GMP (cGMP) are essential messengers that participate in various plant physiological processes, but how these messengers modulate the plant response to N-acyl-homoserine-lactone signals remains poorly understood. Here, we show that the N-3-oxo-decanoyl-homoserine-lactone (3-O-C10-HL), in contrast to its analog with an unsubstituted branch chain at the C3 position, efficiently stimulated the formation of adventitious roots and the expression of auxin-response genes in explants of mung bean (Vigna radiata) seedlings. This response was mimicked by the exogenous application of auxin, H2O2, NO, or cGMP homologs but suppressed by treatment with scavengers or inhibitors of H2O2, NO, or cGMP metabolism. The 3-O-C10-HL treatment enhanced auxin basipetal transport; this effect could be reversed by treatment with H2O2 or NO scavengers but not by inhibitors of cGMP synthesis. Inhibiting 3-O-C10-HL-induced H2O2 or NO accumulation impaired auxin-or 3-O-C10-HL-induced cGMP synthesis; however, blocking cGMP synthesis did not affect auxin-or 3-O-C10-HL-induced H2O2 or NO generation. Additionally, cGMP partially rescued the inhibitory effect of H2O2 or NO scavengers on 3-OC10-HL-induced adventitious root development and auxin-response gene expression. These results suggest that 3-O-C10-HL, unlike its analog with an unmodified branch chain at the C3 position, can accelerate auxin-dependent adventitious root formation, possibly via H2O2- and NO-dependent cGMP signaling in mung bean seedlings.