Morphological and physiological changes induced by contact-dependent interaction between Candida albicans and Fusobacterium nucleatum.
Morphological and physiological changes induced by contact-dependent interaction between Candida albicans and Fusobacterium nucleatum.
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DOI：
10.1038/srep27956
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发表时间：
2016-06-14
影响因子：
4.6
通讯作者：
He X
中科院分区：
文献类型：
--
作者：
Bor B;Cen L;Agnello M;Shi W;He X
白色念珠菌和具核梭杆菌是研究较多的两种口腔微生物，具有潜在的致病性，可引起多种口腔微生物感染性疾病。最近，我们证明了F.具核梭菌ATCC 23726与C.白色念珠菌SN 152的一个主要由梭菌膜蛋白RadD和念珠菌细胞壁蛋白Flo 9介导的过程。本研究的目的是调查这种王国间相互作用的潜在生物学影响。我们发现F.核质ATCC 23726抑制C. albicans SN 152的表达。进一步的分析表明，抑制念珠菌菌丝形态发生是通过RadD和Flo 9蛋白对介导的。使用小鼠巨噬细胞系，我们表明，F。核诱导的念珠菌菌丝形态发生抑制促进C.白念珠菌的生存和负面影响的巨噬细胞杀伤能力的C。白色念珠菌。此外，C. albicans抑制F.核诱导巨噬细胞产生MCP-1和TNFα。我们的研究表明，C。albicans和F.核质导致毒力的相互减弱，这可能起到促进口腔内的长期寄生生活方式的作用。这一发现对我们理解王国间的相互作用具有重要意义，并可能影响临床治疗策略。
Candida albicans and Fusobacterium nucleatum are well-studied oral commensal microbes with pathogenic potential that are involved in various oral polymicrobial infectious diseases. Recently, we demonstrated that F. nucleatum ATCC 23726 coaggregates with C. albicans SN152, a process mainly mediated by fusobacterial membrane protein RadD and Candida cell wall protein Flo9. The aim of this study was to investigate the potential biological impact of this inter-kingdom interaction. We found that F. nucleatum ATCC 23726 inhibits growth and hyphal morphogenesis of C. albicans SN152 in a contact-dependent manner. Further analysis revealed that the inhibition of Candida hyphal morphogenesis is mediated via RadD and Flo9 protein pair. Using a murine macrophage cell line, we showed that the F. nucleatum-induced inhibition of Candida hyphal morphogenesis promotes C. albicans survival and negatively impacts the macrophage-killing capability of C. albicans. Furthermore, the yeast form of C. albicans repressed F. nucleatum-induced MCP-1 and TNFα production in macrophages. Our study suggests that the interaction between C. albicans and F. nucleatum leads to a mutual attenuation of virulence, which may function to promote a long-term commensal lifestyle within the oral cavity. This finding has significant implications for our understanding of inter-kingdom interaction and may impact clinical treatment strategies.