Expression of DNAJA1 in bovine muscles according to developmental age and management factors
Expression of DNAJA1 in bovine muscles according to developmental age and management factors
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DOI：
10.1017/s1751731110002491
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发表时间：
2011-06-01
期刊：
影响因子：
3.6
通讯作者：
Jurie, C.
中科院分区：
文献类型：
--
作者：
Cassar-Malek, I.;Guillemin, N.;Jurie, C.
我们最近发现，编码热休克蛋白（Hsp40）的DNAJA1基因的表达是夏洛来公牛肉嫩度的负标记。为了获得关于DNAJA1表达调控的知识，我们分析了发育过程中DNAJA1转录本和蛋白质的丰度，并根据管理因素（如：G.饲养处理、生长途径和应激状态）对牛肌肉生长的影响。我们在这里报告的DNAJA1的发育表达谱的转录和蛋白质的水平下降，在肌生成的进展。在出生后的生活中，我们发现DNAJA1在最氧化的肌肉中表达最高。未检测到对饮食处理（牧草与玉米为主的饮食）、生长路径（限制期后的补偿性生长）或屠宰前应激状态的影响。因此，遗传背景和肌肉类型可以被认为是影响DNAJA1水平的主要因素。从这项研究中获得的知识的整合应有助于预测肌肉代谢特性和活动物提供高感官质量肉的能力。
We have recently shown that the expression of the DNAJA1 gene encoding a heat shock protein (Hsp40) is a negative marker of meat tenderness in Charolais bulls. To acquire knowledge on the regulation of DNAJA1 expression, we analysed the abundance of DNAJA1 transcripts and protein during development and according to management factors (e. g. feeding treatments, growth path and stress status) in different bovine muscles during postnatal life. We report here a developmental expression profile for DNAJA1 with decreased levels of transcript and protein during the progression of myogenesis. During postnatal life, we found the highest expression of DNAJA1 in the most oxidative muscles. No effect was detected for dietary treatment (pasture v. maize-based diet), growth path (compensatory growth after a restriction period) or pre-slaughter stress status. Therefore, the genetic background and muscle type could be considered as the main factors regarding the level of DNAJA1. Integration of the knowledge gained from this study should help to predict muscle metabolic properties and the ability of the live animals to give high sensory quality meat.