Fast EPR imaging at 300 MHz using spinning magnetic field gradients
Fast EPR imaging at 300 MHz using spinning magnetic field gradients
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DOI：
10.1016/j.jmr.2004.02.012
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发表时间：
2004-06-01
影响因子：
2.2
通讯作者：
Zweier, JL
中科院分区：
文献类型：
--
作者：
Deng, YM;He, GL;Zweier, JL
电子顺磁共振成像（EPRI）技术在过去十年中发展迅速，在生物学和医学领域有许多重要的应用。在300-1200 MHz的频率下，已经进行了一系列体内应用。然而，获取给定数量的投影所需的成像持续时间限制了该技术在其中发生相对快速的动力学的许多体内应用中的使用。因此，有一个很大的需要，以发展的方法，以加快EPRI数据采集。我们报告了一个快速的低频EPRI技术的发展，使用旋转磁场梯度（SMFG）。利用300 MHz CW（连续波）EPRI系统，SMFG实现了10倍以上的图像投影加速采集。2D图像超过200个投影，可以在不到3秒的采集和20秒的采集良好的图像质量上获得大的含水自由基样品。这种技术应该是特别有用的自由基及其代谢的体内研究。(C)2004爱思唯尔公司All rights reserved.
Electron paramagnetic resonance imaging (EPRI) technology has rapidly progressed in the last decade enabling many important applications in the fields of biology and medicine. At frequencies of 300-1200 MHz a range of in vivo applications have been performed. However, the requisite imaging time duration to acquire a given number of projections, limits the use of this technique in many in vivo applications where relatively rapid kinetics occur. Therefore, there has been a great need to develop approaches to accelerate EPRI data acquisition. We report the development of a fast low-frequency EPRI technique using spinning magnetic field gradients (SMFG). Utilizing a 300 MHz CW (continuous wave) EPRI system, SMFG enabled over 10-fold accelerated acquisition of image projections. 2D images with over 200 projections could be acquired in less than 3 s and with 20 s acquisitions good image quality was obtained on large aqueous free radical samples. This technique should be particularly useful for in vivo studies of free radicals and their metabolism. (C) 2004 Elsevier Inc. All rights reserved.