Counterion-paired Bright Heptamethine Fluorophores with NIR-II Excitation and Emission Enable Multiplexed Biomedical Imaging.
Counterion-paired Bright Heptamethine Fluorophores with NIR-II Excitation and Emission Enable Multiplexed Biomedical Imaging.
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DOI：
10.1002/anie.202117436
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发表时间：
2022-03
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通讯作者：
Yang Yang-Yang;Caixia Sun;Shangfeng Wang;Kui Yan;Mengyao Zhao;Bin Wu;Fan Zhang
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文献类型：
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作者：
Yang Yang-Yang;Caixia Sun;Shangfeng Wang;Kui Yan;Mengyao Zhao;Bin Wu;Fan Zhang
光子激发和发射在NIR-II光谱窗口实现高对比度的深部组织生物成像。然而，NIR-II激发和发射的多路复用成像一直受到有限的化学策略的阻碍，以发展明亮的荧光团，在该光谱状态下具有可调谐的吸收。在此，我们通过物理有机方法开发了一系列吸收/发射最大值从1100到1600 nm不等的七甲基菁（hc）。研究发现，与hc配对的大体积对偶离子可显著改善NIR-II菁氨酸在水中的吸收率（7倍）、亮度（14倍）和光谱分布，解决了NIR-II菁氨酸因聚集和极化而导致的臭名昭著的猝灭问题。我们展示了HC1222和HC1342在1120 nm和1319激发下对活小鼠的循环系统、淋巴结构、肿瘤和器官功能进行高对比度双色成像的实用性，使HC1222成为一种有前途的非侵入性生物成像平台。
Photon excitation and emission at the NIR-II spectral window enable high-contrast deep-tissue bioimaging. However, multiplexed imaging with NIR-II excitation and emission has been hampered by the limited chemical strategy to develop bright fluorophores with tunable absorption in this spectral regime . Herein, we developed a series of heptamethine cyanines (HCs) with varied absorption/emission maxima spanning from 1100 to 1600 nm through a physical organic approach. A bulky counterion paired to HCs was found to elicit substantial improvements in absorptivity (7-fold), brightness (14-fold), and spectral profiles in water, addressing a notorious quenching problem of NIR-II cyanines due to aggregation and polarization. We demonstrated the utilities of HC1222 and HC1342 for high-contrast dual-color imaging of circulatory system, lymphatic structures, tumor, and organ function in living mice under 1120 nm and 1319 excitation, enabling HCs as a promising platform for non-invasive bioimaging.