Concentration of copper, iron, zinc, cadmium, lead, and nickel in boar semen and relation to the spermatozoa quality
Concentration of copper, iron, zinc, cadmium, lead, and nickel in boar semen and relation to the spermatozoa quality
复制标题
DOI：
10.1081/ese-120024453
复制
发表时间：
2003-01-01
影响因子：
2.1
通讯作者：
Turcan, J
中科院分区：
文献类型：
--
作者：
Massányi, P;Trandzík, J;Turcan, J
研究了精子中铜、铁、锌、镉、铅、镍的浓度及其与精子质量的关系。采用原子吸收分光光度法（AAS）对精液样品进行分析。猪精液中铜和铁的浓度分别为1.64 +/- 0.28 mg kg和16.14 +/- 10.35 mg kg（-1）。猪精液中锌含量平均值为171.74 +/- 64.72 mg kg(-1)，镉含量平均值为0.05 mg kg(-1)，为0.01 ~ 0.16 mg kg（-1）。分析结果表明，猪精液中铅的含量为0.02 +/- 0.03 mg kg(-1)，镍的平均含量为0.06 +/- 0.08 mg kg（-1）。病理精子总数为9.82±1.47%。详细分析发现：分离鞭毛占3.18%，旋扭鞭毛占2.26%，躯干鞭毛占0.88%，球鞭毛占0.85%，破碎鞭毛占0.42%，胞质滴留0.23%，小头0.14%，大头0.03%，其他病理改变占1.83%。相关分析显示铜与铅呈正相关（p < 0.05） （r = 0.52）。小头与旋钮扭尾（r = 0.67）、小头与断鞭毛（r = 0.88）、小头与病理精子总数（r = 0.73）高度相关。
The concentration of copper, iron, zinc, cadmium, lead, and nickel as well as its relation to spermatozoa quality was investigated. The semen samples were analyzed by atomic absorption spectrophotometry (AAS). The concentration of copper in boar semen was 1.64 +/- 0.28 mg kg(-1) and of iron 16.14 +/- 10.35 mg kg(-1). The concentration of zinc in boar semen reached an average value of 171.74 +/- 64.72 mg kg(-1) and the level of cadmium reached 0.01-0.16 mg kg(-1) with the average value of 0.05 mg kg(-1). The analysis of lead showed that the concentration of this element in boar semen was 0.02 +/- 0.03 mg kg(-1) and the average level of nickel was 0.06 +/- 0.08 mg kg(-1). The total percentage of pathological spermatozoa was 9.82 +/- 1.47%. Detail analysis determined 3.18% of separated flagellum, 2.26% knob twisted flagellum, 0.88% flagellum torso, 0.85% flagellum ball, 0.42% broken flagellum, 0.23% retention of the cytoplasmic drop, 0.14% small heads, 0.03% large heads, and 1.83% forms other of pathological changes. Correlation analysis showed significant (p < 0.05) positive correlation between copper and lead (r = 0.52). High correlation between small head, and knob twisted tail (r = 0.67), small head and broken flagellum (r = 0.88) as well as between small head and total number of pathological spermatozoa (r = 0.73) was determined.