Moxibustion attenuates neurogenic detrusor overactivity in spinal cord injury rats by inhibiting M2/ATP/P2X3 pathway
Moxibustion attenuates neurogenic detrusor overactivity in spinal cord injury rats by inhibiting M2/ATP/P2X3 pathway
复制标题
艾灸通过抑制 M2/ATP/P2X3 通路减轻脊髓损伤大鼠神经源性逼尿肌过度活动
DOI：
10.1016/j.brainres.2022.147926
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发表时间：
2022-05-11
期刊：
影响因子：
2.9
通讯作者：
Liu，Cun-Zhi
中科院分区：
文献类型：
--
作者：
Wang，Lu;Fu，Yuan-Bo;Liu，Cun-Zhi
目的脊髓损伤后，膀胱感觉通路中的M受体的激活被认为是神经源性逼尿肌过度活动的主要原因。本研究是进行检查是否艾灸改善神经性逼尿肌过度活动通过调制异常muscarinic受体pathway.Materials and methodsFemale Sprague-Dawley大鼠进行脊髓损伤与T9-10脊髓横断。14 d后，艾灸治疗1周。测定尿动力学参数和盆腔传入放电。测定排尿液中三磷酸腺苷（ATP）含量。M2，M3，和P2 X3受体在膀胱粘膜的表达进行了evaluated. ResultsMoxidine治疗防止脊髓损伤大鼠逼尿肌过度活动的发展，在收缩间期和排尿压力阈值的增加，并在填充过程中的传入活动减少。艾灸可明显抑制M2的表达，并伴有ATP和P2 X3水平的降低。M2受体拮抗剂methoctramine半水合物有类似的效果，艾灸对膀胱功能和传入活动，而M2-优先激动剂oxotremorine methiodide取消了有益的efficacy of oxization. ConclusionMoxidine是一个潜在的候选人，用于治疗神经源性膀胱过度活动在大鼠模型的脊髓injury，可能通过抑制M2/ATP/P2 X3通路。
PurposeActivation of muscarinic receptors located in bladder sensory pathways is generally considered to be the primary contributor for driving the pathogenesis of neurogenic detrusor overactivity following spinal cord injury. The present study is undertaken to examine whether moxibustion improves neurogenic detrusor overactivity via modulating the abnormal muscarinic receptor pathway.Materials and methodsFemale Sprague-Dawley rats were subjected to spinal cord injury with T9-10 spinal cord transection. Fourteen days later, animals were received moxibustion treatment for one week. Urodynamic parameters and pelvic afferents discharge were measured. Adenosine triphosphate (ATP) content in the voided cystometry fluid was determined. Expressions of M2, M3, and P2X3 receptors in the bladder mucosa were evaluated.ResultsMoxibustion treatment prevented the development of detrusor overactivity in spinal cord injury rats, with an increase in the intercontraction interval and micturition pressure threshold and a decrease in afferent activity during filling. The expression of M2 was markedly suppressed by moxibustion, accompanied by a reduction in the levels of ATP and P2X3. M2 receptor antagonist methoctramine hemihydrate had similar effects to moxibustion on bladder function and afferent activity, while the M2-preferential agonist oxotremorine methiodide abolished the beneficial effects of moxibustion.ConclusionMoxibustion is a potential candidate for treating neurogenic bladder overactivity in a rat model of spinal cord injury, possibly through inhibiting the M2/ATP/P2X3 pathway.