A transcriptomic analysis of the adult stage of the bovine lungworm, Dictyocaulus viviparus.
A transcriptomic analysis of the adult stage of the bovine lungworm, Dictyocaulus viviparus.
复制标题
DOI：
10.1186/1471-2164-8-311
复制
发表时间：
2007-09-05
期刊：
影响因子：
4.4
通讯作者：
Gasser, Robin B
中科院分区：
文献类型：
--
作者：
Ranganathan, Shoba;Nagaraj, Shivashankar H;Hu, Min;Strube, Christina;Schnieder, Thomas;Gasser, Robin B
网尾龙属（网尾龙科）的肺虫是具有重要经济意义的寄生线虫。它们对各种反刍动物宿主，特别是幼年动物，造成病理影响和临床疾病。胎生网尾线虫，又称牛肺线虫，是牛的主要病原体，严重感染可致命。在这项研究中，我们通过分析表达序列标签 (EST) 首次深入了解胎生 D. viviparus 成虫阶段的转录组。使用我们的 EST 分析流程，我们根据 EST 的聚类和比较基因组分析估计，目前的 4436 个 EST 数据集源自 2258 个基因。在 2258 个代表性 EST 中，1159 个（51.3%）在自由生活的线虫中具有同源性，1174 个（51.9%）在寄生线虫中具有同源性，827 个（36.6%）在线虫以外的生物体中具有同源性，863 个（38%）与当前数据库中的任何序列没有显着匹配。在秀丽隐杆线虫同源物中，有 569 个观察到“非野生型”RNAi 表型，包括胚胎致死、母体不育、子代不育、幼虫停滞和生长缓慢。我们可以使用基因本体论 (GO) 对 776 个 (35%) 序列进行功能分类，并与京都基因和基因组百科全书 (KEGG) 中的 696 个 (31%) 序列建立通路关联。此外，我们预测了 85 种分泌蛋白，它们可能代表开发新型驱虫药或疫苗的潜在候选蛋白。对胎生 D. viviparus EST 数据的生物信息学分析阐明了一组相对保守且可能新颖的基因。这项研究中发现的基因应该有助于研究更好地了解胎生 D. viviparus 的基本分子生物学，从长远来看，这可能会导致新的干预策略。胎生 D. viviparus 转录组的表征也为全基因组序列分析和未来的比较转录组分析奠定了基础。
Lungworms of the genus Dictyocaulus (family Dictyocaulidae) are parasitic nematodes of major economic importance. They cause pathological effects and clinical disease in various ruminant hosts, particularly in young animals. Dictyocaulus viviparus, called the bovine lungworm, is a major pathogen of cattle, with severe infections being fatal. In this study, we provide first insights into the transcriptome of the adult stage of D. viviparus through the analysis of expressed sequence tags (ESTs). Using our EST analysis pipeline, we estimate that the present dataset of 4436 ESTs is derived from 2258 genes based on cluster and comparative genomic analyses of the ESTs. Of the 2258 representative ESTs, 1159 (51.3%) had homologues in the free-living nematode C. elegans, 1174 (51.9%) in parasitic nematodes, 827 (36.6%) in organisms other than nematodes, and 863 (38%) had no significant match to any sequence in the current databases. Of the C. elegans homologues, 569 had observed 'non-wildtype' RNAi phenotypes, including embryonic lethality, maternal sterility, sterility in progeny, larval arrest and slow growth. We could functionally classify 776 (35%) sequences using the Gene Ontologies (GO) and established pathway associations to 696 (31%) sequences in Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, we predicted 85 secreted proteins which could represent potential candidates for developing novel anthelmintics or vaccines. The bioinformatic analyses of ESTs data for D. viviparus has elucidated sets of relatively conserved and potentially novel genes. The genes discovered in this study should assist research toward a better understanding of the basic molecular biology of D. viviparus, which could lead, in the longer term, to novel intervention strategies. The characterization of the D. viviparus transcriptome also provides a foundation for whole genome sequence analysis and future comparative transcriptomic analyses.